Dengue virus non-permissive cell lines ?????

Do any one have a list of cell lines that are non-permissive to Dengue virus infection?

09 October 2016 1,830 1 View

Any one have GFP-GFP or dsRED-dsRED plasmid?

03 April 2016 5,548 0 View

Global transcriptional repression; Can I spike in RNA as alternative reference control for sybrgreen real time RT-PCR?

02 March 2016 2,784 4 View

How can I group my Mass spec data according to their biological process, molecular function and cellular component using free Bioinformatics software?

Hi, In my experiment I am interested to find interaction partner of the protein of my interest. For that I tagged my Protein with GFP and a pull down experiment was performed and then I analysed...

10 November 2014 1,969 4 View

What are the criteria to choose an appropriate plasmid to construct a stable cell line for expression of an eukaryotic protein?

Stable cell line.

09 October 2013 8,234 7 View

Stability of extracted RNA?

This is a very fundamental and practical problem faced by most of the lab working with molecular biology, mostly with RNA. I would like to discussion in this scientific forum regarding how most of...

08 September 2012 8,348 43 View

GFP-tagged Protein pulldown; not enough to visualize on 10% PAGE

I want to pull down a membrane bound cytoplasmic GFP tagged protein. For this I ordered GFP tagged beads from chromotech. I am lysing the cells expressing GFP tagged protein of my interest with...

08 September 2012 5,250 5 View

Experimental design for Virus and Host Protein Interaction?

I am working with the apoptosis inhibition by RNA virus. I could figure out the protein in my virus which decelerate the apoptosis in its host. I have also learnt from the literature that it uses...

04 May 2012 1,834 7 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Why are the velocities so high in my Boussinesq approximation Fluent simulation?

Hello, I have a mechanism where air rises due to convective flows. In this simulation, nothing is driving the air except this. I understand that the Boussinesq approximation only works for small...

02 March 2021 4,910 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View