Sujit Pujhari

9 Questions 15 Answers 0 Followers

Questions related from Sujit Pujhari

Dengue virus non-permissive cell lines ?????

Do any one have a list of cell lines that are non-permissive to Dengue virus infection?

10 October 2016 1,901 1 View

Any one have GFP-GFP or dsRED-dsRED plasmid?

Would you like to share if you have double GFP or double dsRED plasmid?

04 April 2016 5,604 0 View

Global transcriptional repression; Can I spike in RNA as alternative reference control for sybrgreen real time RT-PCR?

I am working on a gene expression experiment using SYBR green RT-PCR. I see perfect single peak melting curves for reference gene in my control samples but curves are erratic for treatment sample...

03 March 2016 2,830 4 View

How can I use external standard curve to calculate viral copy no.?

I am using iq5 bio rad real time pcr machine. I want to use the std curve from my previous experiment to analyse my data.

04 April 2015 1,680 2 View

How can I group my Mass spec data according to their biological process, molecular function and cellular component using free Bioinformatics software?

Hi, In my experiment I am interested to find interaction partner of the protein of my interest. For that I tagged my Protein with GFP and a pull down experiment was performed and then I analysed...

11 November 2014 2,040 4 View

What are the criteria to choose an appropriate plasmid to construct a stable cell line for expression of an eukaryotic protein?

Stable cell line.

10 October 2013 8,282 7 View

Stability of extracted RNA?

This is a very fundamental and practical problem faced by most of the lab working with molecular biology, mostly with RNA. I would like to discussion in this scientific forum regarding how most of...

09 September 2012 8,448 43 View

GFP-tagged Protein pulldown; not enough to visualize on 10% PAGE

I want to pull down a membrane bound cytoplasmic GFP tagged protein. For this I ordered GFP tagged beads from chromotech. I am lysing the cells expressing GFP tagged protein of my interest with...

09 September 2012 5,291 5 View

Experimental design for Virus and Host Protein Interaction?

I am working with the apoptosis inhibition by RNA virus. I could figure out the protein in my virus which decelerate the apoptosis in its host. I have also learnt from the literature that it uses...

05 May 2012 1,882 7 View