I am new to SEC and developing this workflow for primarily separating Abeta oligomer from mice brain. However, there are other proteins of interest down the road. I was wondering if anyone can suggest some must-read literature for a beginner.
Thank you for your time!
Best - Navid
Perhaps start here with this free, short article:
- "Introduction to Size Exclusion Chromatography (SEC HPLC)"; https://hplctips.blogspot.com/2013/03/introduction-to-size-exclusion.html
In addition to SEC methods (some have poor resolution), many methods using a C18 or C8 column with wide pores can also be used to retain and resolve many of the key peptides too. When trying to work with clinical Amyloid Beta peptides, the matrix and "other" compounds which are not of interest will guide your separation goals. Try several different modes of chromatography to survey what you have, then let the data point you in the right direction for what to try next.
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