Which protocol do you recommend for the trasfection with pDSRed-MITO vector on adherent cells? In particular how many ug of plasmid do you reccomand to use?
Dear community, I am analyzing a dataset where the outcome variables has a lower-bound of zero, approximately 50% of the values are actually 0, and the other 50% is skewed. In the end, I would...
25 June 2023 4,478 4 View
Hello, I am a synthetic polymer chemist. I would like to explore the simulation counterpart of my experimental work. Can anyone suggest a good software able to simulate polymerization reactions,...
04 February 2021 2,949 4 View
In our lab, sometimes we find some mice that have part of the skull very thin, almost transparent, that it is clearly not well developed. This is particularly a problem when we do stereotaxic...
01 June 2020 8,395 0 View
My research is on critical illness insurance/ compensation among insured. So survey questions for factor influence the uptake or awareness would be best. So far I have go through literature...
23 September 2019 1,551 2 View
Hi everybody, I've started to grow up bacteria in liquid medium in order to set up a growth curve but everytime I try to start it there is this cloud in the middle of the flask. The bacteria...
26 March 2018 4,819 2 View
I am looking for diagnostics tool for communication of deaf children or hearing impairment children, including spoken and sign languae. I have found just polish diagnostic tool called KOZK and...
22 January 2018 2,249 3 View
I have a binary mixture of two molecules able to establish hydrogen bonds and with similar volume. One of the pure has a viscosity LARGELY lower than the other, so I would expect that adding the...
19 March 2017 5,897 4 View
I have a bunch of binary mixtures of two solvents (i.e. water and ethanol) of different composition, from water mol.frac. 0 to 1. For each of them I have the experimental density. Now I want to...
31 December 2016 4,201 3 View
In the canonical use of the Arrhenius law (i.e. kinetics), the activation energy is related to the height of the reaction barrier and the pre-exponential factor is related to the probability of...
01 September 2016 7,796 6 View
Dear network, I am co-editing the Special Issue "Model Predictive Control-Based Approach for Microgrids" with deadline on 20 July 2024. If you are interested in the topic, please, checkout the...
01 January 1970 6,661 0 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
Hi all, I need to introduce an ARS (autonomously replicating sequence) in my plasmid but I'm not sure which position would be the best. Does anyone have any suggestion? A picture of the plasmid...
05 August 2024 1,573 4 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....
05 August 2024 2,570 3 View
Dear Researchers I need to know, how to load and plot 2D-PIV (particle image velocimetry) recorded velocity vector field data in Tecplot? Thank You
02 August 2024 3,615 1 View
Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...
31 July 2024 9,892 4 View
Hello I am trying to create a stable cell line in HEK293 via Lipofectamine 3000 transfection. My plasmid is a CD63-IL10-GFP construct with Puromycin resistance. I am successful with the...
30 July 2024 6,648 1 View
I have been trying to electroporate SKOV3 cells with a large plasmid (11kb) without much success. Any tips?
29 July 2024 3,229 1 View
I created two potential gene expression cassettes (constitutive and inducible) for expression of a mutant PETase gene on PeptiCloud using the version tree feature, which allows users to create...
28 July 2024 7,559 1 View
Please address the best way to drop a plasmid. Background: I have a "bait" plasmid resistant to kanamycin and a "prey" plasmid resistant to carbenicillin. After many rounds of streaking on...
25 July 2024 2,532 3 View