Hi everybody,
I've started to grow up bacteria in liquid medium in order to set up a growth curve but everytime I try to start it there is this cloud in the middle of the flask.
The bacteria strain is a gram-negative, aerobic and non-motile in liquid.
Growth condition are: 200ml broth in 1L flask 37°C degrees with 200rpm speed.
I've tried to plate it and the colture seem to be uncontaminated
Any suggestion?
Thanks