Hi everybody,

I've started to grow up bacteria in liquid medium in order to set up a growth curve but everytime I try to start it there is this cloud in the middle of the flask.

The bacteria strain is a gram-negative, aerobic and non-motile in liquid.

Growth condition are: 200ml broth in 1L flask 37°C degrees with 200rpm speed.

I've tried to plate it and the colture seem to be uncontaminated

Any suggestion?

Thanks

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