facing problem to separate acid and its acid anhydride on HPLc
Hi Yogesh,
it depends on your sample matrix and how complex is it. the first thing i would try is anion exchange - the acid should bind but not the anhydride.
Cheers,
David.
Dear Yogesh:
In what solvent have your analytes?
Thanks.
Best Regards,
Áurea Andrade-Eiroa
Please Explain more? Is it aromatic or aliphatic acids?
used Tio2 as etc and mixed peroskite as the active layer . but upon heat treatment the phase is same . i.e. doesn't turn yellow . what could be the possible reason ?
08 November 2023 4,926 0 View
"Many of the papers have left readers perplexed when attempting to replicate the device due to insufficient or unclear experimental details. Can you recommend a paper that provides more...
05 November 2023 2,018 0 View
please me some article related to my topic, and suggest me about the same. it will be a great help.
06 August 2023 770 4 View
the research topic "decsion making in career planning within IT industry using AI" in department of computer application is correct, What i have to do for this.
24 July 2023 1,722 3 View
Dear All, I am working on Rapid test development (Lateral flow based). I am facing conjugate release issue after few months of strip preparation. Up to approx 30 days, conjugates release found...
20 July 2023 8,648 8 View
Gut microbiome study requires the quantification of bacterial load before and after antibiotic treatment. for which DNA is isolated from faeces. It would be grateful if the calculation could be...
21 June 2023 2,482 0 View
A prototype of the MsM harvesting device with Metglas 2605SC laminate bonded on a cantilever beam wound by a pick-up coil. Explanation of formula "Um = Metglas layer strain energy" Paper:...
17 June 2023 6,310 2 View
I am trying to calculate the LOD of electrochemical sensor, used for heavy metal ions detection. i know , LOD= 3Sb/s , where Sb is the standard deviation of blank solution (without analyte) and s...
16 June 2023 735 1 View
Hello All, I am working on E. coli and want to study its gene expression profile using qPCR. I grew the culture till stationary phase and snap freeze the cell pellet using liquid nitrogen. I...
20 April 2023 7,280 4 View
Since in electrolysis of water in alkaline medium, Availability of OH- ion is more so Oxygen evolution reaction should not be more sluggish so in this way HER should be more sluggish? Please...
06 December 2022 2,012 2 View
A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...
11 August 2024 9,801 2 View
Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...
11 August 2024 8,141 3 View
Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!
11 August 2024 3,770 4 View
Hello experts, Does anyone know any free software about retention index prediction ?
08 August 2024 7,403 2 View
I'm currently working on calculating the collision cross section (CCS) for various ions, and I'm facing challenges when dealing with sodiated and multiply charged ions. Most of the resources I’ve...
08 August 2024 8,329 0 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.
07 August 2024 8,782 3 View
Are there any suggestions or insights you can provide to help address this problem
06 August 2024 1,443 2 View
I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...
06 August 2024 1,367 3 View
Mobile apps can be a powerful tool for enhancing academic performance, how can we use mobile apps for improving academic performance
04 August 2024 9,492 0 View