Gut microbiome study requires the quantification of bacterial load before and after antibiotic treatment. for which DNA is isolated from faeces. It would be grateful if the calculation could be simplified for the same purpose.
4-Dimethyl amino pyridine is used in reaction.Product formed contains amino group along with amide & is highly water soluble. To remove 4-DMAP aqueous work up done. Please give solution. Thanks.
21 February 2021 1,883 1 View
any SCI,SCIE, and Scopus index ( or simillar) journals of relevant field
17 December 2020 7,148 4 View
I have XRD data of a doped powder sample. How can I perform the Rietveld refinement using GSAS-2. When I use cif file of doped powder, symmetry is lost in the refining process. How to perform...
10 December 2020 9,717 4 View
Greetings, I have a short stretch of 20 aa with strong binding affinity for a compound. I am trying to de some whole cell flourescence or visible biosensing technique based on this peptide. Can...
26 November 2020 8,322 3 View
Can i check superlattice period for tio2 thin film made via spin coating ? superlattice period is seen only in MBE or can be there in tio2 coated on glass via spin coating ??
20 September 2020 3,748 3 View
Can we use the graphite moderator rods from nuclear waste in the mini reactor ,beta voltage cell decay ? or we can use the thermo-electric generator is there any possibility? for some miniature...
15 September 2020 1,726 8 View
i have got the following photo luminescence spectra data of AU-PD tio2 film for diffrent depotion rates ,can some one help me to analyse it broadly? can we discuss it ??
02 September 2020 3,260 6 View
I have deposited on glass surface SrTiO3 . I want to deposit TiO2 over it via spin coating for further studies . Will I observe any difference in properties ? or Can I use this substrate for...
24 August 2020 6,654 3 View
I have created new material in ANSYS Workbench with parameters obtained from the experimental tension test. I have provided the data of Stress strain, E, Syt, Sut, density, poissions ratio while...
13 August 2020 5,458 5 View
Hi, I am studying a transporter protein. X ray crystallographic structure is not known yet. So, i am using homolgy modelled structures for my studies. I am curious if i should put position...
30 July 2020 3,191 1 View
and Can the gut microbe be used for the treatment or diagnosis of liver fibrosis?
02 March 2021 557 2 View
based on what I've studied on multiple papers, for peptide and protein identification Alpha-cyano-4-hydroxycinnamic acid (CHCA) is an appropriate matrix to use, I also know that the CHCA powder...
26 February 2021 6,799 3 View
Hi, I'm looking for universal primers targeting a bacterial mRNA to quatify bacterial load by qPCR in a complexe mixture (eukaryote and prokaryote RNA). Ribosomal RNA being depleted, I absolutly...
22 February 2021 9,969 3 View
When dealing with bacterial toxins, there are three varieties of microbes that develop in your petri dish: toxic, resistant, and susceptible. Initially, there are only toxic and vulnerable...
21 February 2021 6,084 5 View
I am working on the MIC determination of various antibacterial agents (both water-soluble and- insoluble) against S. aureus, MRSA, and S. pyogenes. Some guidelines recommend using round-bottomed...
17 February 2021 7,029 3 View
I have been reading various articles and most state that treatment with CaCl2 produces higher efficiency of transformation than MgCl2. Can anyone explain why Ca2+ is better than Mg2+ at...
16 February 2021 8,519 5 View
Dear colleagues, I have an output of FUNGuild tool (ASVs abundance in different samples and assigned to guilds) and would like to merge the ASVs that had similar ecological guilds using R...
16 February 2021 4,292 7 View
Hi Fellow Scientists! I am trying to clone my 300 bp insert into a 6 kb vector. I run a few different ligation reactions using (1:3 vector: insert molar ratios) 10, 20,30, and 40 ng/ul vector...
15 February 2021 9,563 6 View
Hello all, I am interested in deleting a highly expressed non-coding RNA located on a Listeria monocytogenes plasmid. Currently, there are not any optimized gene deletion methods for Listeria...
09 February 2021 4,994 1 View
This is a follow-up on an earlier question regarding mechanistics of vaccines: why current vaccines don't block transmission. In summary, they don't engage mucosal immune cells to produce IgA....
08 February 2021 8,321 3 View