Does it mandatory to show all the synthesis with the same procedure in review article...?

When we are writing a review article if I want to add the previous synthesis of a particular molecule...but there are so many papers with the same procedure on that molecule...does it mandatory to...

18 November 2020 1,109 4 View

How to deplete MDSCs in mice bearing 4T1 tumors?

07 September 2016 8,375 4 View

How can I stably transfect 4T1 with gene of interest?

30 November 2015 4,941 4 View

What is relationship between TCID50 and viral nucleic acid nquantitation? Does is tell us the absolute quantity of virus in the sample?

what does TCID50 tell us?

16 July 2014 8,925 3 View

Can TCA precipitation be done with samples already in 1x SDS sample buffer?

I had solubilized organellar pellet with (1%SDS, 0.1N NaOH) and resolved in gel in presence of 1x SDS sample buffer, but the separation of the proteins does not look that good. I would like to...

17 December 2013 3,291 3 View

Research papers for ATM card fraud pattern detection

Is it possible to detect the real time ATM card fraud detection using data warehouse based system. I've upto one million transaction dataset in the Oracle database & I need to prepare a fraud...

15 July 2013 1,839 1 View

Which algorithm can be good for ATM Card Fraud pattern detection?

We've about 1 million ATM transaction records and need to separate the fraudulent transactions from the whole data set. I've studied about Support Vector Machine (SVM) algorithm, ANN (BPN),...

28 June 2013 9,776 8 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

No title

02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View