I am looking for anti GluA1 antibody to perform immunofluorescent staining for surface receptor in cultured neurons.
Hello
See papers here hope help you .
Good Luck
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3240854/
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4775841/
http://www.biocompare.com/pfu/110447/soids/614330/Antibodies/GluA1
Hi Jivan,
The N-terminal anti-GluA1 rabbit antibody (#PC246) from Calbiochem gives strong surface labeling in cultured neurons. The staining (using 1:30 dilution) looks punctate and predominant on dendrites than cell body.
Good luck !
Hi Dhrubajyoti.
Thanks a lot.
Hello Researcher, I would like to ask a question about crude (1H) NMR product yield calculation? When we assign product integration and internal standard integration for percentage yield...
30 April 2022 6,504 4 View
Does anyone know how to find out whether crystal structure has been published or not..? I have synthesized reported molecules by different methodology and got a single crystal. I tried to find out...
27 August 2021 1,398 5 View
I am going to do blue-led light setup for photochemical reaction....what kind of light source should I use...? some scientist using blue lead bulb, some are making manually using ordinary small...
30 May 2021 7,595 3 View
When we are writing a review article if I want to add the previous synthesis of a particular molecule...but there are so many papers with the same procedure on that molecule...does it mandatory to...
18 November 2020 1,300 4 View
I did some experiments infecting Salmonella Typhi with the lytic phage I isolated from the environment. It gives a nice clear plaques on agar overlay. However, in tube co-culture growth assay, the...
08 November 2019 5,035 5 View
We are going to make methyl salicylate, which catalyst best for 100 % conversion.
19 December 2018 7,795 4 View
I am working on FMD virus by RT-PCR . I took a trivalent attenuated vaccine as a reference. for universal primers it gave positive but for serotyping was negative even though its trivalent vaccine...
11 November 2016 5,827 2 View
I want to know the protocols to deplete MDSCs in mice bearing 4T1 tummors with particular emphasis on : 1. Which regimen of MDSC depletion (anti-Gr1 or Gemcitabine??) is better? 2. Dose and...
07 September 2016 8,538 4 View
Does the IRES-eGFP in the bicistronic construct be in-frame with the gene of interest upstream of it? I put my gene of interest upstream of EMCV-IRES-EGFP and realized that EGFP is in frame 2...
02 April 2016 3,930 4 View
I want to be able to control the expression of protein of interest in 4T1 cell line and wanted to check if there could be a threshold of expression that determines the efficacy of vaccination...
30 November 2015 5,065 4 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...
07 August 2024 5,338 2 View
To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...
06 August 2024 6,728 6 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
Hi everyone, I am working on brain slices for visualizing a protein in the soma and dendrites, using a fluorescence tag. However, I need a tool (not paid) for reconstruction of the whole neuron,...
04 August 2024 4,725 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
I am currently looking for a questionnaire that relates with my study which is The Effectiveness of Organizational Culture towards employee retention rates
03 August 2024 3,505 6 View