Any good alternative to ApoTox to asses viability/cytotoxicity/apoptosis?

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Lorenz Waltl

MTT assay and FACS

Stephan Permann

If you have coloured compounds, you could also use trypan blue staining to check the toxicity.

They might - but don't have to - interfere with the MTT readout.

Yuan Ma

I suggest testing cell viability and cytotoxicity with CCK8 kit and cell apoptosis with TUNEL staining. Both two techniques are not complicated and easy to get started.