I do not quite understand your question: are you thinking of L-form bacteria (no cell wall)? https://en.wikipedia.org/wiki/L-form_bacteria , Article Cell wall-deficient, L-form bacteria in the 21st century: A ...

These can be generated from normal E.coli by growing them under conditions where peptidoglycan synthesis is inhibited, or by treatment with lysozyme. However, the L-forms are very sensitive to osmotic stress.

If not, can you be more specific? You can certainly not abolish all function of proteins intrinsic to the plasma membrane, as the bacteria need transport proteins to take up nutrients.

For knock-outs of any one specific protein (including membrane and cell wall proteins), look at the Keio Knock-out collection (https://cgsc2.biology.yale.edu/Scope.php)

Annemarie Honegger Actually i need that

E.coli strain whose all surface proteins mutated (cell wall/plasma membrane proteins) for my research, i mean mutated surface protein e.coli strain.

Thanks a lot Annemarie Honegger

Noor Ul Akbar