15 Questions 14 Answers 0 Followers
Questions related from Ramiz Raza
Does different microscope measure different width(e.g. x, y and z rather than x only) of a particular device having only x width at the same objective?
20 April 2021 6,038 2 View
Is any way or method to seal agarose pad or channels fabricated agarose gel pad to glass slides as like we do plasma bonding with PDMS to glass slides?.
05 January 2021 3,817 2 View
Can we transform any gfp tagged plasmid vector in to any E.coli strains with a objective to visualize E.coli bacteria as fluorescence molecule?. I need a valuable suggestions so Please help me....
01 December 2020 2,645 8 View
Could anyone give valuable inputs of this strain nomenclature F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), λ-, ΔpotD783::kan, rph-1, Δ(rhaD-rhaB)568, hsdR514?. Actually I need only pot D mutant...
24 November 2020 6,430 5 View
How can i get nonsporulating bacterial bacillus subtillis strain Δ spoIIE without antibiotic growth conditions ? This ΔspoIIE::erm strain is showing growth conditions with antibiotic...
11 November 2020 7,326 4 View
Could any one say how to detect calcium concentration or calcium flux out of cell but not within the cell? Please also help me for calcium indicator for the same.
30 September 2019 7,011 5 View
If photoresist is coated over first alignment marks then how will I do 2nd alignment through MJB4 mask aligner?
15 August 2019 2,414 5 View
I want 1000 bacteria (numbers) to begin my experiments .How will i get these numbers? Any specific methods? please give suggestions.
05 August 2019 8,087 10 View
How to track multiple particles or bacteria in ImageJ? I am using multitracker in imageJ but unable to track many particles and bacteria. please help and give valuable suggestions.
27 July 2019 1,837 5 View
Could anybody say what is the general porosity of PDMS , i mean pore size ? can we use PDMS for DNA run?? one more thing what is the pore size of Whatmann filter paper??
08 April 2019 1,841 2 View
How do we decide or calculate exposure time for particular thickness of SU8 resist while doing photolithography? Kindly share your experience as well as valuble suggestions.I will be thankful to you.
20 March 2019 5,728 5 View
How do make a glycerol stock of bacteria for future use? I need valuable suggestions for that.Please put here and share your experience.
26 February 2019 9,452 3 View
Fluorescent intensity of cytosolic GFP of bacterial cell is assumed to be equal to their cell volume? what does it mean?. could anybody explain it why cytosolic GFP? and their intensity...
18 February 2019 2,351 4 View
Can I fabricate same height of main channel(h=5um) and narrow channels(5um) which is joint between two main channel(5um) through multlayer lithography?.kindly give valuable suggestions.thanks
08 February 2019 7,428 10 View
I mean it would be agar surface down or up?
22 August 2018 9,225 15 View
