Direct interspecies electron transfer
@Stephanie please trying the NCBI site, here you can blast for primer or at least related primers where you can now begin optimization.
Hello, I am new to designing flow cytometry experiments. I have some cells transfected with EGFP using mRNA and want to quantify its percentage expressed. I also wish to differentiate between...
17 May 2024 8,527 2 View
Hello RG community, I am looking for remote sensing data on cloud cover estimation on a daily basis but during nighttime over Namibia /Southeast Africa. The ideal spatial resolution would be 1km...
31 March 2024 298 2 View
I am currently doing isolation of protein from inclusion bodies. I solubilize the protein with 8M guanidine hydrochloride (Simpson, CSHL, 2010). I this step I saw no pellet anymore in my sample....
17 March 2024 8,573 0 View
How to form the immune complex using human IgG?
16 November 2023 3,811 1 View
I want to study the male reproductive system for two different seasons to obtain histology, histometery, morphology and morphometery. Please guide me how much should be my simple size. What would...
22 May 2023 7,177 5 View
I am working on my dissertation, and I am stuck on the type test I should run on my data. Research Design-I have a control and a treatment group where a vocabulary intervention was given to the...
02 April 2023 1,263 4 View
I am trying to do a shRNA gene silencing for an esophageal cell line. Does anyone have any specific protocol to suggest?
27 March 2023 6,675 3 View
I performed a reaction to modify BSA through esterification, but it didn't seem to work. Most of the papers that I've found that attach azide to BSA seem to use maleimide. Is there a reason why...
09 March 2023 4,971 2 View
policies initiatives during past few years
08 March 2023 5,540 2 View
How do we as scientists interpret data to provide the most explicit explanation for our research? I am aware that we can present the limitations of our study which can defer our...
15 February 2023 2,867 1 View
I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?
11 August 2024 3,115 1 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
If we map as a continuous motion an ionising electron (beginning its journey at n=1) in an H atom, a specific hyperbolic spiral appears (see animation). When we solve this spiral formula, we find...
07 August 2024 5,343 2 View
I have modelled a steel structure using beam elements in Abaqus and attached to this structure reinforced concrete slab. The analysis that I am making is heat transfer of the structure. The...
07 August 2024 1,028 0 View
Hello dear colleagues, We have prepared a manuscript on NiTi-based alloys and are seeking a second opinion on our current TEM results. If you are a Ph.D. holder with experience in TEM and have...
07 August 2024 9,563 0 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
How to design VN primer to attach with universal reverse primer
05 August 2024 2,116 3 View
average particle size calculation from TEM
04 August 2024 2,921 1 View
If from a geometric perspective the non-halogens, non-noble gases have more empty spots in their valence shell, and the filling/exiting of any of the empty spots in the shell constitutes a...
03 August 2024 4,787 2 View
Why is the molecule's orientation with an electric field affect polarizability? Electrons are diffuse enough to be independent with respect to orientation and effect of electric field on...
03 August 2024 7,843 1 View