Any advice to measure NK cells activity in spleen?

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Can anybody give me a protocol to measure NK cell activity?

Hi everyone, I'm working with immunosuppressed mouse model. Now I treated mice with cyclophosphamide (immunosuppressive drugs), so I need a protocol to measure the NK cell activity after...

31 December 2018 6,037 5 View

Cyclophosphamide (Cy) and Scid mice !!

Hello everyone, I am trying to produce immunosuppressed mice in my protocol so I want to understand the difference between scid mice and cyclophosphamide mechanism. Any suggested please?

05 June 2017 5,926 8 View

Minigenome assay for polymerase activity

Hello everyone, some one are familiar with technique from Minigenome assay for polymerase activity Any suggested please?

05 June 2017 3,227 2 View

Is it necessary to confirm the RNA-seq results by quantitative RT-PCR?

Hello, Im working with co-infection PRRS virus/Swine influenza virus, i'm waiting for RNA-seq data, so please I need to know Is it necessary to confirm the RNA-seq results by quantitative RT-PCR...

07 August 2016 3,535 4 View

About co-infection between PRRS and PCV virus ?

Hello I need to introduce some mutation in clone ciro-virus, for studying the effect of co-infection between PRRS and PCV virus (pigs) on cells replication please I need help about this topic,...

08 September 2015 7,282 0 View

What - what brings epidemiology to the practice of veterinary public health?

Hello What - what brings epidemiology to the practice of veterinary public health?

03 April 2015 5,207 4 View

They are scientists sites to criticize Articles ?

Hello, I want to know a site where I can see reviews of scientific articlesthank you

11 December 2014 4,144 1 View

Does formalin inactivate the Influenza virus ?

Hello I am trying to find a method to inactivate swine influenza viruses that are produced in eggs, most of the items gives formalin, but I find a specific protocol, I need suggestions and ideas...

10 November 2014 7,916 17 View

How can I detect mutation sites on Epitope HA?

Hello I am trying to genetically characterize strains of influenza virus, and after sequencing, I do my alignments CLUSTER W, and I get the phylogenetic trees for each gene. I want to detect...

08 September 2014 2,981 3 View

Can anyone help with genetic and antigenic variability in the Influenza virus?

hello I work on the genetic and antigenic variability of influenza virus in swine. Is the observed variability be due to PCR or are they all real? Why do my MDCK cells and eggs are mainly used to...

08 September 2014 7,575 4 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

The Bigger You Are, the Harder You Fall (some lessons from Dinosaurs)?

Evolutionary fitness is based on an organism’s ability to adapt rapidly to changing environmental circumstances. Large-bodied mammals have been equipped with large brains (and hence a high...

06 August 2024 4,849 2 View

Are air moisture harvesting technologies effective in combating desertification?

Air moisture harvesting Air water collection devices

06 August 2024 5,473 2 View

How to determine positive-stained cells in FACS? Use isotype or unstained control?

To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...

06 August 2024 6,728 6 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

Why do we equate male and female arousal?

Women, on the other hand, can become physically aroused (increased blood flow in the reproductive organs) without becoming psychologically aroused even in the slightest. (Robert Weiss)

05 August 2024 9,537 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to use Density Functional Theory to calculate carrier mobilities of solid system?

Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...

04 August 2024 8,894 1 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View