Hello
I need to introduce some mutation in clone ciro-virus, for studying the effect of co-infection between PRRS and PCV virus (pigs) on cells replication please I need help about this topic, some one working with the same strategy
Thank you
Hi everyone, I'm working with immunosuppressed mouse model. Now I treated mice with cyclophosphamide (immunosuppressive drugs), so I need a protocol to measure the NK cell activity after...
31 December 2018 6,037 5 View
Hi everyone, I would like to know a good marker ( flow cytometry) for measure the NK cell activity in spleen ? Thank you in advance
09 October 2018 618 2 View
Hello everyone, I am trying to produce immunosuppressed mice in my protocol so I want to understand the difference between scid mice and cyclophosphamide mechanism. Any suggested please?
05 June 2017 5,926 8 View
Hello everyone, some one are familiar with technique from Minigenome assay for polymerase activity Any suggested please?
05 June 2017 3,227 2 View
Hello, Im working with co-infection PRRS virus/Swine influenza virus, i'm waiting for RNA-seq data, so please I need to know Is it necessary to confirm the RNA-seq results by quantitative RT-PCR...
07 August 2016 3,535 4 View
Hello What - what brings epidemiology to the practice of veterinary public health?
03 April 2015 5,207 4 View
Hello, I want to know a site where I can see reviews of scientific articlesthank you
11 December 2014 4,144 1 View
Hello I am trying to find a method to inactivate swine influenza viruses that are produced in eggs, most of the items gives formalin, but I find a specific protocol, I need suggestions and ideas...
10 November 2014 7,916 17 View
Hello I am trying to genetically characterize strains of influenza virus, and after sequencing, I do my alignments CLUSTER W, and I get the phylogenetic trees for each gene. I want to detect...
08 September 2014 2,981 3 View
hello I work on the genetic and antigenic variability of influenza virus in swine. Is the observed variability be due to PCR or are they all real? Why do my MDCK cells and eggs are mainly used to...
08 September 2014 7,575 4 View
Pig slurry is rich in major and minor nutrients. Is there any way to improve / Enrich its manure quality to be used in agriculture organically ? please share your knowledge.
09 August 2024 5,605 2 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
I am trying to simulate vehicular loading on an orthotopic steel deck bridge section in ABAQUS software. The red arrow mark in the attached figure indicates the direction in which the vehicle will...
08 August 2024 719 0 View
Hi, we have measured tryptic peptides using both DDA and DIA method on QExactive. In DDA replicates i saw unusual intensity drops occurring at the same sections of chromatograms in DDA replicates...
07 August 2024 3,218 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Hi, everyone. I want to transfer two genes in to the pseudomonas bacteria that isolated from the soil. The bacteria that I want to use for cloning haven't been identified and not be sequenced,...
02 August 2024 3,987 1 View
Hello, We found three packages of Illustra™ MicroSpin™ G-25 columns in the cabinet of an unused lab. They are very old but have never been opened. I have never used this kit before, and I couldn't...
25 July 2024 4,927 3 View
I am trying to put 3 separate plasmids into Staphylococcus aureus and I am looking for those that are theta replicating and compatible. I have obtained pSK9065 (from pSK1) from Addgene, but the...
23 July 2024 6,968 0 View
I am cloning an overexpression plasmid with my protein of interest tagged with mScarlet. After transforming my ligated product into DH5α bacteria and plating on LB agar, I noticed colonies with a...
22 July 2024 5,953 6 View
I have immunized BalB/C mice with a protein using the intradermal (ID) method with Complete Freund's Adjuvant (CFA) and Incomplete Freund's Adjuvant (IFA), following a 14-day interval and three...
22 July 2024 9,160 2 View