About co-infection between PRRS and PCV virus ?

09 September 2015 0 7K Report

Hello

I need to introduce some mutation in clone ciro-virus, for studying the effect of co-infection between PRRS and PCV virus (pigs) on cells replication please I need help about this topic, some one working with the same strategy

Thank you

Badges
Science topic

More Zeineb Mhamdi's questions See All

Can anybody give me a protocol to measure NK cell activity?

Hi everyone, I'm working with immunosuppressed mouse model. Now I treated mice with cyclophosphamide (immunosuppressive drugs), so I need a protocol to measure the NK cell activity after...

31 December 2018 6,037 5 View

Any advice to measure NK cells activity in spleen?

Hi everyone, I would like to know a good marker ( flow cytometry) for measure the NK cell activity in spleen ? Thank you in advance

09 October 2018 618 2 View

Cyclophosphamide (Cy) and Scid mice !!

Hello everyone, I am trying to produce immunosuppressed mice in my protocol so I want to understand the difference between scid mice and cyclophosphamide mechanism. Any suggested please?

05 June 2017 5,926 8 View

Minigenome assay for polymerase activity

Hello everyone, some one are familiar with technique from Minigenome assay for polymerase activity Any suggested please?

05 June 2017 3,227 2 View

Is it necessary to confirm the RNA-seq results by quantitative RT-PCR?

Hello, Im working with co-infection PRRS virus/Swine influenza virus, i'm waiting for RNA-seq data, so please I need to know Is it necessary to confirm the RNA-seq results by quantitative RT-PCR...

07 August 2016 3,535 4 View

What - what brings epidemiology to the practice of veterinary public health?

Hello What - what brings epidemiology to the practice of veterinary public health?

03 April 2015 5,207 4 View

They are scientists sites to criticize Articles ?

Hello, I want to know a site where I can see reviews of scientific articlesthank you

11 December 2014 4,144 1 View

Does formalin inactivate the Influenza virus ?

Hello I am trying to find a method to inactivate swine influenza viruses that are produced in eggs, most of the items gives formalin, but I find a specific protocol, I need suggestions and ideas...

10 November 2014 7,916 17 View

How can I detect mutation sites on Epitope HA?

Hello I am trying to genetically characterize strains of influenza virus, and after sequencing, I do my alignments CLUSTER W, and I get the phylogenetic trees for each gene. I want to detect...

08 September 2014 2,981 3 View

Can anyone help with genetic and antigenic variability in the Influenza virus?

hello I work on the genetic and antigenic variability of influenza virus in swine. Is the observed variability be due to PCR or are they all real? Why do my MDCK cells and eggs are mainly used to...

08 September 2014 7,575 4 View

How to enrich pig excreta for increasing nutrient quality organically ?

Pig slurry is rich in major and minor nutrients. Is there any way to improve / Enrich its manure quality to be used in agriculture organically ? please share your knowledge.

09 August 2024 5,605 2 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

How can I apply boundary conditions in an orthotropic steel deck numerical model using ABAQUS software?

I am trying to simulate vehicular loading on an orthotopic steel deck bridge section in ABAQUS software. The red arrow mark in the attached figure indicates the direction in which the vehicle will...

08 August 2024 719 0 View

Unusual intensity drop in some sections of chromatograms in DDA?

Hi, we have measured tryptic peptides using both DDA and DIA method on QExactive. In DDA replicates i saw unusual intensity drops occurring at the same sections of chromatograms in DDA replicates...

07 August 2024 3,218 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

How can we now if the promotor in the vector is well?

Hi, everyone. I want to transfer two genes in to the pseudomonas bacteria that isolated from the soil. The bacteria that I want to use for cloning haven't been identified and not be sequenced,...

02 August 2024 3,987 1 View

Illustra™ MicroSpin™ G-25 columns what it is used for?

Hello, We found three packages of Illustra™ MicroSpin™ G-25 columns in the cabinet of an unused lab. They are very old but have never been opened. I have never used this kit before, and I couldn't...

25 July 2024 4,927 3 View

Does anyone know a source for theta-replicating Staphylococcus plasmids that are compatible?

I am trying to put 3 separate plasmids into Staphylococcus aureus and I am looking for those that are theta replicating and compatible. I have obtained pSK9065 (from pSK1) from Addgene, but the...

23 July 2024 6,968 0 View

Pink bacterial colonies?

I am cloning an overexpression plasmid with my protein of interest tagged with mScarlet. After transforming my ligated product into DH5α bacteria and plating on LB agar, I noticed colonies with a...

22 July 2024 5,953 6 View

What are the strategies to Enhance IgG-Producing Plasma Cells in mice for Monoclonal Antibody Development?

I have immunized BalB/C mice with a protein using the intradermal (ID) method with Complete Freund's Adjuvant (CFA) and Incomplete Freund's Adjuvant (IFA), following a 14-day interval and three...

22 July 2024 9,160 2 View