Can anybody give me a protocol to measure NK cell activity?

Hi everyone, I'm working with immunosuppressed mouse model. Now I treated mice with cyclophosphamide (immunosuppressive drugs), so I need a protocol to measure the NK cell activity after...

31 December 2018 5,950 5 View

Any advice to measure NK cells activity in spleen?

09 October 2018 536 2 View

Cyclophosphamide (Cy) and Scid mice !!

05 June 2017 5,846 8 View

Is it necessary to confirm the RNA-seq results by quantitative RT-PCR?

07 August 2016 3,458 4 View

About co-infection between PRRS and PCV virus ?

08 September 2015 7,203 0 View

What - what brings epidemiology to the practice of veterinary public health?

Hello What - what brings epidemiology to the practice of veterinary public health?

03 April 2015 5,088 4 View

They are scientists sites to criticize Articles ?

Hello, I want to know a site where I can see reviews of scientific articlesthank you

11 December 2014 4,071 1 View

Does formalin inactivate the Influenza virus ?

Hello I am trying to find a method to inactivate swine influenza viruses that are produced in eggs, most of the items gives formalin, but I find a specific protocol, I need suggestions and ideas...

10 November 2014 7,764 17 View

How can I detect mutation sites on Epitope HA?

08 September 2014 2,905 3 View

Can anyone help with genetic and antigenic variability in the Influenza virus?

08 September 2014 7,463 4 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View