Dear Zeinab,

Maybe you can look here :

http://blog.addgene.org/minigenomes-a-safe-way-to-study-dangerous-viruses

and here:

http://www.sciencedirect.com/science/article/pii/S1684118214000383

To catch a glimps of what this technique is all about.

Maybe here they are talking also a bit about minigenomes:

http://pubmedcentralcanada.ca/pmcc/articles/PMC2801414/

I had courses some times ago in this field, but I never had the chance to use it.

Hope it helped,

Regards,

Mathieu

Thank you so much Mathieu