Greetings,
I am currently sorting a rare population of cells by flowcytometry. Getting very small amount of the sorted cells in a volume of 5 ml (including sheath buffer and 2%FBS/PBS). Tried so hard to make a pellet from this 5 ml. But in some reason, couldn't see any pellet and losing a lot of cells in supernatant. Follwoing my yield of DNA is speechlessly small.
Can anyone provide some way to get over this situation?
how could i get a cell pellet without losing
with regards
Min