5 Questions 13 Answers 0 Followers
Questions related from Hyo-Min Kim
Greetings, I am currently sorting a rare population of cells by flowcytometry. Getting very small amount of the sorted cells in a volume of 5 ml (including sheath buffer and 2%FBS/PBS). Tried so...
12 December 2015 6,550 13 View
I keep trying Western Blotting using lysozyme antibody from SC and abcam, however I couldn't detect the band. Is there any suggestion that I can get through this? I used donkey anti-goat for a...
10 October 2015 2,803 3 View
Used HBSS ( containing Ca2+ and Mg2+) + 2% FBSS solution for binding antibody. However, all my result seems kind of awkward. Can 2+ ion effect on binding efficiency? or Not? help me through...
08 August 2015 179 5 View
Any good explanation for Sanger way and NGS of genome sequencing?
07 July 2015 7,132 3 View
I saw some protocol that used diluted Matrigel (1 to 1 Matrigel with DMEM/F12.) for epithelial cell cultrue. I know that diluted matrigels are used for plate coating, however is there someone who...
06 June 2015 6,133 4 View