Hyo-Min Kim

5 Questions 13 Answers 0 Followers

Questions related from Hyo-Min Kim

Any advice in pelleting from FACS sorted cells ?

Greetings, I am currently sorting a rare population of cells by flowcytometry. Getting very small amount of the sorted cells in a volume of 5 ml (including sheath buffer and 2%FBS/PBS). Tried so...

12 December 2015 6,547 13 View

I keep trying Western Blotting using lysozyme antibody (from Santa Cruz and abcam), however I couldn't detect the band. Any suggestions?

I keep trying Western Blotting using lysozyme antibody from SC and abcam, however I couldn't detect the band. Is there any suggestion that I can get through this? I used donkey anti-goat for a...

10 October 2015 2,801 3 View

Does Ca2+,Mg2+ ion base buffer(such as HBSS with 2+ ions) affect antibody binding?

Used HBSS ( containing Ca2+ and Mg2+) + 2% FBSS solution for binding antibody. However, all my result seems kind of awkward. Can 2+ ion effect on binding efficiency? or Not? help me through...

08 August 2015 176 5 View

What is main difference in sequencing method between Sanger way and NGS ?

Any good explanation for Sanger way and NGS of genome sequencing?

07 July 2015 7,130 3 View

What is the appropriate way to use diluted Matrigel for epithelial cell culture?

I saw some protocol that used diluted Matrigel (1 to 1 Matrigel with DMEM/F12.) for epithelial cell cultrue. I know that diluted matrigels are used for plate coating, however is there someone who...

06 June 2015 6,129 4 View