Hi,

the most important and general difference is that Sanger sequencing results represent population data i.e. the consensus sequence of a sample and NGS results are based on single molecule data.

Hello,

There are many difference between this approaches

Sanger methods achieve read lengths of approximately 800 bp. NGS methods allow to obtain full genomes. And many many other.

There are many references about it.

http://www.esciencecentral.org/ebooks/applications-of-molecular-genetics/dna-sequencing-sanger-and-nextgeneration-sequencing.php

Or in the Wikipedia.

https://en.wikipedia.org/wiki/DNA_sequencing#Next-generation_methods

https://en.wikipedia.org/wiki/Sanger_sequencing

You could read previously discussion here.

https://www.researchgate.net/post/What_are_the_differences_between_pyrosequencing_and_sequencing_by_the_Sanger_method

Thank you all!