Hello all,
I isolate HUVECs for my research.
Kindly suggest me an CD31 marker product code (from Thermo scientfic or Sigma aldrich) for immunofluorescence microscopic characterization of HUVECs.
Thank you so much.
Dear Jyotsana,
I think the following product could be very efficient for your analysis: CD31 monoclonal antibody (MA3100). You can access to the product by this link: https://www.thermofisher.com/antibody/product/CD31-Antibody-clone-HEC7-Monoclonal/MA3100
Best regards,
Dave
Hi Jyotsana,
For characterization of HUVEC by CD31 immunofluorescence staining I would recommend using polyclonal (e.g. rabbit) anti-CD31 Ab with a FITC (or any other fluorochrome) labelled matching secondary Ab. Alternatively, you may use acetylated-LDL in live cell culture to check the purity/characterize your isolated HUVEC (if that's the reason you are using CD31immunofluorescence staining approach).
Best
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