ELISA for testing a custom made EGFR antibody?

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Bacteria growing well on nutrient broth but not on NA plates. What could be reason? How can it be resolved?

I am facing a problem. I have isolated few bacteria from soil and preserved them in glycerol stock (50 % glycerol v/v). Now when I am inoculating these cultures in nutrient broth and incubating...

06 December 2020 3,577 6 View

Should outlier analysis be done before PCA while trying to perform step-wise Regression? Also how to deal with outliers?

Currently I am working on a project wherein I need to predict the estimated new Selling price of IPL players based on their current base price and previous years' performance & sold price. I'm...

05 August 2020 4,254 6 View

How could we create an innovative learning environment?

In teaching we use different techniques and tools for making it interesting but how can we make it more innovative and creative?

19 July 2020 1,567 34 View

Use cases of Internet of Things based solution for COVID-19?

We have identified a use case of IoT (Quarantine people tracking) (paper link)IoT-Q-Band: A low cost internet of things based wearable ban... Is there any other indicative application related...

04 May 2020 8,263 34 View

How to transfer bacteria from the agar plate to liquid medium?

detailed description of the process

12 August 2019 4,411 3 View

Can anyone help me with determining protein expression using quantitative Real time PCR?

I am working on a sperm specific protein, I want to determine its concentration in control and infertile. Can it be done using real-time PCR and how?

24 June 2019 9,292 9 View

How to understand MATLAB codes of Ant colony optimization (ACO) for travelling salesman problem (TSP)?

I am a beginner in MATLAB, and I am finding it very difficult to understand sample codes and the functions used in it even after googling "help (that function)". How should I approach to...

12 April 2019 8,396 4 View

What are some topics of research under Industry 4.0 for production and industrial engineers?

What are some topics related to production and industrial engineers?

07 April 2019 2,312 14 View

Are some research topics inferior in comparison to others among researchers?

What do you think?

05 April 2019 4,913 7 View

What could be a possible fabrication technique to prepare dense polycermets (Polymer+Ceramic+Metal based composites) ?

The designed composite could have ideal properties from each system and could be used for some targeted applications.

01 April 2019 4,260 4 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View