ELISA for testing a custom made EGFR antibody?

More Nidhi Jyotsana's questions See All

How can we conduct DFT for solutions or mixtures ?

Can we conduct DFT studies for Ionic Liquids with Amines at various concentrations? If so, how can we proceed with these studies? Additionally, what parameters, besides IR, NMR, and HOMO-LUMO, can...

18 July 2024 319 1 View

How rise in NPAs leads to high efficiency in private sector banks in India?

Please guide me how MPAs has positive and significant relation with efficiency in Indian Private banks while NPAs are negativity and insignificantly related to efficiency in public and foreign...

27 February 2024 6,654 0 View

How employee productivity is insignificantly related to efficiency in private banks while significantly related in public & foreign banks in India?

I want to analyze the relationship between efficiency and income diversification in Indian banks. Employee productivity (Business per employee) is a control variable in regression that is...

25 February 2024 9,389 0 View

Can constant/intercept be insignificant in regression model if all other conditions are satisfied?

13 February 2024 8,194 5 View

Can fractional logistic regression be used in two stage DEA analysis? (In this technical efficiency is my dependent variable, bounded between 0 to 1)?

In most of the studies tobit regression is used but in tobit model my independent variable is not significant. Whether fractional logistic regression is also an appropriate technique to explore...

01 February 2024 4,641 2 View

What is new in Menstrual Health and Hygiene in terms of marketing?

I am researching the taboo behavior around women's menstrual health and hygiene, and exploring ways to shift it towards marketing through better research design.

16 December 2023 2,530 1 View

How is the expression pattern of Lon Protease 1 (LonP1) of mitochondria during the course of differentiation in mouse embryonic stem cells?

Does the expression of LonP1 decreases with differentiation?

09 October 2023 9,012 0 View

Why grain size decrease after aging? does precipitates and grain both move together in aging process? if yes then what is driving force ?

Basically I want to know why grain boundary becomes small after aging ,as at temperature increases grain size.

17 August 2023 5,744 0 View

Does the shape of the notch affect the fracture test result?

Generally in fracture test pre cracking takes some time which depends on materials but in my case the pre cracking of an Al alloy sample with square notch is completing very soon like in 10...

16 August 2023 6,855 4 View

What are the best and reliable gel filtration based-spin columns to purify DNA and RNA ?

I am trying to fluorescently label RNA and purifying the labeled RNA further. The protocol recommends using gel filtration based membrane in the spin columns.

10 August 2023 2,332 1 View

Mouse CD3 antibody sequence?

Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much

08 August 2024 9,157 4 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

How to make a Lateral flow control line more uniform?

We have a lateral flow test on Sartorius 140 NC. The conjugate is gold-monoclonal antibody. Using different control lines (GAM, protein A, protein G) we get a very strong leading edge with reduced...

31 July 2024 4,510 3 View

What is the weight of rotavirus A (ng)?

I am developing an ELISA kit for rotavirus A (G1P8 and G1P6). Does anyone know the weight (ng) of these two strains? Or to help me find their weight?

29 July 2024 3,138 2 View

Why my IgG antibody looks fragmented on non-reducing SDS-PAGE?

I am performing IgG purification and I have to show my results on SDS-PAGE. I use 10% tris glycine gel and prepare the samples under non reducing conditions. I am new to antibodies and therefore...

23 July 2024 6,664 6 View

What are the strategies to Enhance IgG-Producing Plasma Cells in mice for Monoclonal Antibody Development?

I have immunized BalB/C mice with a protein using the intradermal (ID) method with Complete Freund's Adjuvant (CFA) and Incomplete Freund's Adjuvant (IFA), following a 14-day interval and three...

22 July 2024 9,160 2 View

What are those black/hollow structures?

Hello, I recently stained my cells with an Arf6 antibody. The healthy control looks pretty normal, while the disease group cells have abnormally large black structures in the cell body. Has anyone...

21 July 2024 1,044 1 View

How to activate 4E8 T cells with plate-bound anti-CD3 for a genome-wide CRISPR screen?

It was assumed that 4E8 T cells should be isolated and activated using plate-bound anti-human CD3, after which would be used for a genome-wide CRISPR screen. (Ref: Genome-wide CRISPR Screens in...

17 July 2024 3,371 0 View

ELISA sandwich with two monoclonal antibodies ?

hi, I am facing difficulties in the développement of an ELISA sandwich. I have several experiences in developing ELISA but that’s the first time I can’t find any solution. My test is currently as...

16 July 2024 9,044 10 View

Questions of antibodies selection?

Hi there, I'd like to use immunocytochemistry to determine a surface protein expression on mouse cell. The primary antibody I have is mouse anti mouse, and the secondary antibody I have is goat...

15 July 2024 6,008 5 View