Bacteria growing well on nutrient broth but not on NA plates. What could be reason? How can it be resolved?

I am facing a problem. I have isolated few bacteria from soil and preserved them in glycerol stock (50 % glycerol v/v). Now when I am inoculating these cultures in nutrient broth and incubating...

06 December 2020 3,577 6 View

Should outlier analysis be done before PCA while trying to perform step-wise Regression? Also how to deal with outliers?

Currently I am working on a project wherein I need to predict the estimated new Selling price of IPL players based on their current base price and previous years' performance & sold price. I'm...

05 August 2020 4,254 6 View

How could we create an innovative learning environment?

In teaching we use different techniques and tools for making it interesting but how can we make it more innovative and creative?

19 July 2020 1,567 34 View

Use cases of Internet of Things based solution for COVID-19?

We have identified a use case of IoT (Quarantine people tracking) (paper link)IoT-Q-Band: A low cost internet of things based wearable ban... Is there any other indicative application related...

04 May 2020 8,263 34 View

How to transfer bacteria from the agar plate to liquid medium?

detailed description of the process

12 August 2019 4,411 3 View

Can anyone help me with determining protein expression using quantitative Real time PCR?

I am working on a sperm specific protein, I want to determine its concentration in control and infertile. Can it be done using real-time PCR and how?

24 June 2019 9,292 9 View

How to understand MATLAB codes of Ant colony optimization (ACO) for travelling salesman problem (TSP)?

I am a beginner in MATLAB, and I am finding it very difficult to understand sample codes and the functions used in it even after googling "help (that function)". How should I approach to...

12 April 2019 8,396 4 View

What are some topics of research under Industry 4.0 for production and industrial engineers?

What are some topics related to production and industrial engineers?

07 April 2019 2,312 14 View

Are some research topics inferior in comparison to others among researchers?

What do you think?

05 April 2019 4,913 7 View

What could be a possible fabrication technique to prepare dense polycermets (Polymer+Ceramic+Metal based composites) ?

The designed composite could have ideal properties from each system and could be used for some targeted applications.

01 April 2019 4,260 4 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

No title

02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

No title

28 February 2021 9,936 2 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View