Normalization luciferase data assay with number of cells?

Hello everyone, I am running luciferase assay with pMIR REPORT plasmid to evaluate the function of a miRNA on a specific 3'UTR. I wondering if I could normalize the firefly signal to the number of...

06 July 2017 2,190 3 View

Wrong p53 size on Western blot?

Hi I am blotting for p53 in my experiment. The predicted MW is 53 kDa. However the actual MW appears to be higher than the expected MW in SDS-PAGE. I loaded 10 micro-gram protein. I didn't have...

06 July 2017 7,401 5 View

Is is possible to knock out miRNA with antagomir?

Hi all, I appreciate if you could help me answer this question. Our lab is interested in the role of one miRNA and would like to know the effect of depletion of this miRNA. Is it possible to knock...

05 June 2016 7,836 2 View

Identify cysteine residue that is responsible for disulfide bond formation?

04 May 2016 7,500 4 View

Can anyone tell me how to identify targeted proteins pertaining to apoptosis pathway?

Hi everyone, I am doing a project to predict and prioritize miRNAs and their targets that are involved in regulating apoptosis. We have already had the results of miRNAs expression and their...

01 February 2016 5,148 3 View

Is anyone familiar with postranscriptional regulation?

09 October 2014 4,425 10 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View