Nghiem Le

7 Questions 3 Answers 0 Followers

Questions related from Nghiem Le

Wrong p53 size on Western blot?

Hi I am blotting for p53 in my experiment. The predicted MW is 53 kDa. However the actual MW appears to be higher than the expected MW in SDS-PAGE. I loaded 10 micro-gram protein. I didn't have...

07 July 2017 7,438 5 View

Normalization luciferase data assay with number of cells?

Hello everyone, I am running luciferase assay with pMIR REPORT plasmid to evaluate the function of a miRNA on a specific 3'UTR. I wondering if I could normalize the firefly signal to the number of...

07 July 2017 2,261 3 View

Is is possible to knock out miRNA with antagomir?

Hi all, I appreciate if you could help me answer this question. Our lab is interested in the role of one miRNA and would like to know the effect of depletion of this miRNA. Is it possible to knock...

06 June 2016 7,879 2 View

Identify cysteine residue that is responsible for disulfide bond formation?

Hi all, I am doing a small project in which I see that a mutant in protein A, that changes arginine to cysteine, can form a disulfide bond with another cysteine protein B. The problem is that...

05 May 2016 7,538 4 View

Antibody against pro-caspase 3 also recognizes same position of beta-actin?

Hi all, I am testing the anti-apoptotics effects of a miRNA on H9c2 cell lines, in specific I focus on capsase 3. After 48 hours tranfecting H9c2 cells with mimic and antagomir, I collected cell...

02 February 2016 4,357 3 View

Can anyone tell me how to identify targeted proteins pertaining to apoptosis pathway?

Hi everyone, I am doing a project to predict and prioritize miRNAs and their targets that are involved in regulating apoptosis. We have already had the results of miRNAs expression and their...

02 February 2016 5,186 3 View

Is anyone familiar with postranscriptional regulation?

Can any body help me explain whether the following statement is true or false? If the site of transcript cleavage and polyadenylation of a particular RNA in one cell is downstream of the same...

10 October 2014 4,462 10 View