Antibodies to mTurquoise?

More Hugo Alonso-Cantabrana's questions See All

Are the coordinates the center of the cell or a corner in GADM / worldclim maps in R?

I am currently using a map downloaded from https://gadm.org/maps/DEU/bayern.html and I would like to assign the value of a variable to each cell with R. I was also trying to get this information...

15 July 2024 8,844 1 View

How can i generate a CRISPR knockin mutation zebrafish model with a reporter?

Hey! I aim to generate a transgenic knockin zebrafish line that mimetizes a genetic condtition that leads to a certain disease on human. To do so, I need to insert a codon for mutagenic aminoacid...

14 July 2024 6,240 0 View

Path analysis: Do I control for covariate effects on all endogenous variables (and mediator) or just DV?

I did research and the studies suggest controlling for the effects of covariates on all endogenous variables, including mediator variables, to improve model accuracy and reliability. I want to...

04 June 2024 1,914 3 View

Does anyone have the manual for the PBCON-24T-700-4 lighting controller?

Good afternoon, everyone. I am trying to locate the manual of a lighting controller, whose code is PBCON-24T-700-4 from the company DesignInnova or Photo Biosim. Does anyone know how to get it or...

28 May 2024 8,240 0 View

Leg debris for identification ?

Good morning, I am trying to identify the species to which this leg belongs. Especially its biogeographic origin, namely whether it could be a species from France or China. Thank you for any...

07 April 2024 1,461 0 View

How to count cells of a filamentous cyanobacteria with a Neubauer chamber ?

I would like to work with Nostoc punctiforme, which is filamentous, and try to assess its growth by cell counting using a Neubauer chamber. Does anyone have experience with this? Is it possible to...

24 March 2024 1,906 0 View

Which is your experience with immersive virtual reality in medical education?

I would like to know what is your experience with immersive virtual reality in training programmes for nurses or doctors. If you have tried any immersive virtual reality simulator please send me...

03 March 2024 5,056 10 View

Literature: how do energy interests use energy crises?

I am looking for literature on how different interests (e.g. energy companies, governments, political parties, industries, business associatons, etc) use energy crises and concerns over energy...

31 January 2024 4,248 0 View

Tol2 - Is it normal to lose the transgene expression after a few days?

I'm using a transposon tol2 vector (pT2AL200R150G - 10.1534/genetics.106.060244) to create transgenic zebrafish line. We first inserted our gene of interest (GOI) into the BamHI site of the...

21 November 2023 9,618 1 View

Where can I find the raw data for S-N curve of steels?

Dear fellow researchers, I would like to know where I can find the editable/raw data for a set of points of a S-N curve for a high strenght steel, like a dual phase. I can only find the S-N...

19 November 2023 8,866 3 View

Does anyone know what might be causing the smeared bands on my western blot?

I got these smeared bands quite often lately. We typically run the gel at 140V with a 10-12% gel and do a wet transfer at 220 mA for 1.5 hr in cold room. We also noticed some dirty spots/dots (see...

10 August 2024 7,480 3 View

Mouse CD3 antibody sequence?

Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much

08 August 2024 9,157 4 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

What is the best blank for nanodrop if I want to read a recombinant protein concentration?

Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC

01 August 2024 967 3 View

How to make a Lateral flow control line more uniform?

We have a lateral flow test on Sartorius 140 NC. The conjugate is gold-monoclonal antibody. Using different control lines (GAM, protein A, protein G) we get a very strong leading edge with reduced...

31 July 2024 4,510 3 View

Problems with ion exchange chromatography?

I'm having problems with ion exchange chromatography. After applying my sample (a fluorescent protein), a preferential path inevitably forms. The matrix I use is DEAE-Sephacel. Could someone...

27 July 2024 6,605 0 View

How do regenerate Amberlite XAD 4 resin for further cycles of use for removing 0.5% Sarkosyl from recombinant protein solutions?

I use 0.5% sarkosyl to solubilise recombinant proteins contained in Inclusion bodies of E. coli. sarkosyl very effeciently solubilizes proteins from Inclusion bodies and I am trying to use...

25 July 2024 5,001 2 View

Why my IgG antibody looks fragmented on non-reducing SDS-PAGE?

I am performing IgG purification and I have to show my results on SDS-PAGE. I use 10% tris glycine gel and prepare the samples under non reducing conditions. I am new to antibodies and therefore...

23 July 2024 6,664 6 View

How do nutrient transporters and aquaporins interact under abiotic stress in plants?

"The Correlation between Nutrition and Transport Mechanism under Abiotic Stress in Plants: A Comprehensive Review" by Muhammad Saleem, Jianhua Zhang, Muhammad Qasim, Rashid Iqbal, and Li Song,...

23 July 2024 9,109 0 View

What are the strategies to Enhance IgG-Producing Plasma Cells in mice for Monoclonal Antibody Development?

I have immunized BalB/C mice with a protein using the intradermal (ID) method with Complete Freund's Adjuvant (CFA) and Incomplete Freund's Adjuvant (IFA), following a 14-day interval and three...

22 July 2024 9,160 2 View