Hi,

In the last 3 months we are struggling with recurrent contaminations in our cell cultures. PCR results showed that the bacteria are Sphingopyxis sp. FD7 (by sequence alignment). Although I used several antibiotics that worked nicely as cocktail (Gentamycin+Nanomycopulitine+PSN), the bacteria usually are coming back a few days after I stop antibiotics. I treat the cells usually 2-4 weeks.

Does anybody know where can I look for data of antibiotics sensitivity? Or what is a possible source for this contamination (human? water bath? other?)?

I would appreciate any information that might help to get rid of this contamination.

Thanks in advance,

Orit