Could you please share your experience with me on the type of 96 wells plate for MIC determination: Treated Vs Untreated and Round-Vs Flat-bottomed?

I am working on the MIC determination of various antibacterial agents (both water-soluble and- insoluble) against S. aureus, MRSA, and S. pyogenes. Some guidelines recommend using round-bottomed...

17 February 2021 7,029 3 View

Hi everyone, I want to lower the pH of microbiological broth, what are the best options?

I will be using the lowered pH broth for MIC test of some disinfectants, and I do not want the acid to have any interfernece with MIC results.

03 February 2021 6,959 4 View

How to interpret the result of agar well diffusion assay using herbal extract?

I performed the antibacterial activity of isolated herbal extract against Rhodococcus equi and used Erythromycin as a standard drug, ZOI of the plant extracts were 16-20mm and Erythromycin was 28...

17 January 2021 1,746 3 View

How can I prepare the erythromycin stock?

I need to prepare a stock of 10 mg / ml erythromycin to check the mic value. I am trying to dissolve 0.1g/10 ml of water but i can't dissolve erythromycin in water. How can i prepare 10 mg/ml...

03 January 2021 9,947 3 View

How many ratio of simplicia powder : water I should add to make the infusion concentration 100%?

I am doing research to test antibacterial activity using plant leaves that I have mashed. How many ratio I should add between the simplicia and water to get 100% concentration in infusion technique?

06 December 2020 1,010 6 View

What is the most controllable (marine) bacterial culture you can cultivate in simulated seawater?

02 December 2020 6,796 3 View

My question is about how to determined MIC concentration in the use of microdilution method?

I would like to ask you about MIC first, after I searching in the internet about my query I could not found any answer. My question is about how can I calculate the concentration of stock...

23 November 2020 6,737 3 View

Are 96-well plate adhesive seals necessary in broth microdilution MIC assays?

I am going to perform a minimum inhibition concentration (MIC) assay using the broth microdilution method which will involve a 12-16 hour incubation period of the 96-well plate in a shaking...

19 November 2020 5,928 3 View

Can I make mixture of cinnamic acid with morin flavanoid ?

I am working on a project for assessing the Antibacterial activity of various chemicals, I was wondering Can i make solution of those components using water as a solvent, depending on the fact...

19 November 2020 7,857 4 View

Is DI water appropriate for use as a solvent in MIC testing?

I am performing a minimum inhibitory concentration (MIC) test using the broth microdilution method. The compounds I am testing are polar which means I could dissolve my compounds in distilled...

04 November 2020 7,652 2 View