What non-parametric alternative can I use for a repeated-measures ANOVA for 2 groups and 2 timepoints?

Hello, I have a question regarding non-parametric alternatives for a RM-ANOVA. In my study I had two groups that were measured on two timepoints (pre-post measurements only). I was planning to...

11 February 2021 8,599 7 View

[Sand Lizards tracked with VHF telemetry] How do I analyze the results of my home range estimations properly?

Hello, I just recently started working with the different types of home range estimations and have a few questions about this and that. Maybe you could help me out with that?! My study animals are...

03 February 2021 5,573 3 View

What is the best way to elicit personal narratives in children with SLI/DLD?

Hi all, I am conducting research into the narrative abilities of children with DLD. Apart from standardized tests, we want to elicit some personal narratives from the children to have a good...

05 November 2019 1,455 1 View

How to preserve the BBB during mice irradiation ?

Hello ! I want to perform bone marrow transplantation on mice. First of all I want a total ablation of the BM of the recipient. I plan to achieve this using irradiation. I want to preserve the...

14 February 2019 1,597 2 View

Is it possible to use the Standardized Precipitation Evapotranspiration Index (SPEI) R package with matrices?

I would like to calculate SPEI using the R package developed by the authors. There seems to me to be two major reasons why I can't use the R package (I'm currently trying to figure out SPEI in...

10 May 2017 10,004 2 View

Any who has experience with epigenetic services from Diagenode??

18 February 2016 9,446 3 View

I am considering using structural equation modelling to help understand causality in an agroecosystem? Is this an appropriate approach?

Structural equation modelling (SEM) is not commonly used in ecology or specifically in the agricultural sciences. I am interested in determining causal factors affecting agroecosystem function...

09 October 2015 2,547 6 View

Searching for telemetry studies on Corn bunting

Hi all, Corn buntings are declining very rapidly. By that, we will figure out where their breeding site(s) are from our winter population Corn bunting. For that, we tried to use color rings/...

01 January 1970 924 1 View

How vaccines of covid 19 work?

Millions ask how vaccines of covid 19 be successful and safe, its here to explain generally how vaccine of pfizer work ,mRNA vaccines teach our cells how to make a protein that will trigger an...

28 February 2021 2,723 4 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Best plasmid could be used as a standard curve for AAV titration?

I have been trying to have a good AAV titration for my production and I have tried different plasmids to set up as a standard curve but each one behaves differently. i am trying to use ITR for...

23 February 2021 5,167 3 View

How to control the variants vof covid 19 in second and third waves?

A the world find means to control covid 19 by vaccination ,new variants came to spread and make the world in fear and danger, to understand the conduct of these variants, es constantly change...

21 February 2021 3,080 6 View

Surprisingly low antibody titers against SASRS-COV-2 RBD in hamsters compared to mice?

21 February 2021 6,958 2 View

Will SARS-CoV-2 Spike be broken into S1 and S2 by denaturing conditions?

Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...

17 February 2021 4,844 4 View

How to calculate the TCID50 when the highest CPE is 50% or less?

I am doing TCID50 to measure progeny virus and some treatments protected the cells in a way that the highest CPE observed is 50% or less. How should I do the titre calculation in that case? Thank you!

16 February 2021 8,768 1 View

How to estimate/know plant virus concentration to make a standard curve in ELISA after serial dilution?

Hello, I am trying to know tomato virus titer/concentration in samples and I will perform serial dilution of the positive control. I wanted to ask How to estimate/know plant virus concentration to...

13 February 2021 6,534 4 View

What are the possible effects of COVID -19 Vaccine/s on the persons already recovered from the disease?

Persons recovered from COVID -19 are having antibodies against the virus inside their bodies. They are having T cells already sensitized to the antigens of the virus. If they are vaccinated (may...

12 February 2021 4,106 22 View

What is the reason, we got both test and control band on LFA in negative sample?

I am working on Lateral flow assay for virus detection by carbon conjugated polyclonal antibody. We got a high intense band in both test and control line on LFA in negative sample used as buffer....

11 February 2021 6,558 2 View