Hi,
Due to a colleague suddenly leaving without updating his files/ labjournal we are currently unable to properly continue with the TaqMan PCR research he was performing as we do not know which primers and probes he mixed together to come to the current version of the mix he was using in his research.
We still have access to the original primer/ probe test mix and know which locus it is targeting but the exact composition is now lost to us but we want to identify the individual components used in this mix.
Identifying the primes will be relatively easy (just sequence PCR product) but how should we go about identifying the probe component in this mix?
Any ideas that will not involve GMO / cloning work (no license for this).
Do all of your oligo orders go through a central person/department to the company that makes the oligos/probes in which case they may have a record of the purchase order. If you knew the name of the probe then customer support at the company can usually find the information sheets for orders going to any account. Your lab manager may help in this situation. An order number might be helpful if your accounts dept can print out an expenditure sheet for his grant money
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