Do you have any information on the cloning of a gene pET vector and its expression in BL21?

Dear All, A gene has been cloned in pET alpha 28 to express in BL21. The protein is unable to express. What might be the problem? How can the sequence cloned be verified by any suitable software...

06 July 2014 3,816 8 View

Benzimidazole resistance in Haemonchus contortus (sheep/goat nematode)?

Some laboratories are working on finding benzimidazole drug resistance against haemonchosis in sheep. Workers are finding b-tubulin gene mutation at p200 through gene sequencing. They are actually...

04 May 2014 1,329 1 View

What statistical software program is needed to draw graphs and conclusion?

The file is attached for the question cited above 'What statistical software program is needed to draw the graphs and conclusion'

06 July 2013 2,258 44 View

Is there commercially available cell lines of lymphocyte or tick cells to propagate merozoite or sporozoite stage of protozoa

I am looking for a lymphocyte cell line that supports the development of merozoites and a tick cell line for the support of sporozoites of theileria.

01 February 2013 2,035 5 View

Advantages of using SAS software

Does anybody have experience using SAS software and share its advantages over other softwares like SPSS or Graphpad prism 5 or Stat R? I want to use it for biological research.

06 July 2012 4,344 12 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View

Benefits of the 24 welled plate?

We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...

28 February 2021 1,149 1 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View

Can I use a protein-devoid condition as my negative control in ELISA?

Hello, I am running a sandwich-type ELISA where I am trying to inhibit a protein-protein interaction using small molecules. For each experiment, I determine my Z' (Z-prime) to determine the...

23 February 2021 7,429 1 View

Surprisingly low antibody titers against SASRS-COV-2 RBD in hamsters compared to mice?

21 February 2021 6,958 2 View

Can anyone advise me about a cortisol antibody made in rabbit to set up an ELISA?

I am trying to set up a cortisol EIA using mouse anti-rabbit IGg as coating and a rabbit cortisol antibody as secondary ab. I am searching for a sensitive and specific cortisol Ab made in rabbit

20 February 2021 3,366 5 View