Anti-bovine conjugated secondary antibodies

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Do you have any information on the cloning of a gene pET vector and its expression in BL21?

Dear All, A gene has been cloned in pET alpha 28 to express in BL21. The protein is unable to express. What might be the problem? How can the sequence cloned be verified by any suitable software...

06 July 2014 3,902 8 View

Benzimidazole resistance in Haemonchus contortus (sheep/goat nematode)?

Some laboratories are working on finding benzimidazole drug resistance against haemonchosis in sheep. Workers are finding b-tubulin gene mutation at p200 through gene sequencing. They are actually...

04 May 2014 1,427 1 View

What statistical software program is needed to draw graphs and conclusion?

The file is attached for the question cited above 'What statistical software program is needed to draw the graphs and conclusion'

06 July 2013 2,349 44 View

Is there commercially available cell lines of lymphocyte or tick cells to propagate merozoite or sporozoite stage of protozoa

I am looking for a lymphocyte cell line that supports the development of merozoites and a tick cell line for the support of sporozoites of theileria.

01 February 2013 2,120 5 View

Advantages of using SAS software

Does anybody have experience using SAS software and share its advantages over other softwares like SPSS or Graphpad prism 5 or Stat R? I want to use it for biological research.

06 July 2012 4,453 12 View

What is the weight of rotavirus A (ng)?

I am developing an ELISA kit for rotavirus A (G1P8 and G1P6). Does anyone know the weight (ng) of these two strains? Or to help me find their weight?

29 July 2024 3,138 2 View

ELISA sandwich with two monoclonal antibodies ?

hi, I am facing difficulties in the développement of an ELISA sandwich. I have several experiences in developing ELISA but that’s the first time I can’t find any solution. My test is currently as...

16 July 2024 9,044 10 View

What do I do with dried urine samples?

I placed mice in metabolic cages to collect urine samples for analysis. However, the majority of the samples have dried up. Is there any way to retrieve the urine in those tubes without...

14 July 2024 6,724 0 View

How do I prepare rat cardiomyocyte cell lysate for ELISA via freeze-thaw method?

Anyone have a protocol for making cell lysate from rat cardiomyocyte pellets to run an ELISA using PBS as the buffer?

23 June 2024 2,264 4 View

What is considered a biological replicate when you are doing an ELISA plate measurement with different cell types?

If cells obtained from different passages, with different plating but harvested on the same day are used in a single plate to take an ELISA measurement (with the given standards and treated...

17 June 2024 6,705 1 View

Different interpolated concentrations?

I used two standards (one came with the ELISA kit and the other I made myself, by preparing a series of known concentrations of the target analyte) on the same plate in my ELISA run. Why are the...

15 June 2024 5,109 1 View

Question about ELISA with the "Cattle IGF 1 (Insulin like growth factor) ELISA Kit" Cat: ELK5630?

Dear fellow researchers, We are conducting an ELISA with the "Cattle IGF 1 (Insulin-like growth factor) ELISA Kit" (Cat: ELK5630). Samples and reagents were prepared following the instruction...

13 June 2024 9,473 0 View

How to convert mIU/mL of beta galactosidase enzyme to pg/ml? I have used ELISA to determine beta gal concentration in mIU/ mL in human serum samples?

We have to report the concentration in pg/ml, the ELISA kit provider did not reveal the specific activity of the enzyme. In that case how to convert the units?

05 June 2024 2,173 5 View

What is Area Under the Curve for detecting SARS-COV-2 neutralizing antibodies?

I have a question about AUC for calculating SARS-CoV-2 neutralizing antibody tests. AUC here is not the Area Under the ROC Curve. After six serial dilutions of human serum, an indirect ELISA was...

05 June 2024 8,191 0 View

Can I dilute the serum sample further than stated in the manual as long as the analyte concentration remains within the KIT linear range?

I want to measure a protein in serum using an ELISA kit. This kit allows serum samples to be diluted 200 to 3200 times, but I want to use this kit to titrate higher concentrations of protein. Can...

03 June 2024 4,937 0 View