Do you have any information on the cloning of a gene pET vector and its expression in BL21?

Dear All, A gene has been cloned in pET alpha 28 to express in BL21. The protein is unable to express. What might be the problem? How can the sequence cloned be verified by any suitable software...

06 July 2014 3,816 8 View

Benzimidazole resistance in Haemonchus contortus (sheep/goat nematode)?

Some laboratories are working on finding benzimidazole drug resistance against haemonchosis in sheep. Workers are finding b-tubulin gene mutation at p200 through gene sequencing. They are actually...

04 May 2014 1,329 1 View

What statistical software program is needed to draw graphs and conclusion?

The file is attached for the question cited above 'What statistical software program is needed to draw the graphs and conclusion'

06 July 2013 2,258 44 View

Anti-bovine conjugated secondary antibodies

Does anybody have experience of using anti-bovine secondary antibody conjugated with enzyme? I want to use it in ELISA for the sero-diagnosis of parasitic diseases in cattle and buffalo. Does...

06 July 2012 5,797 2 View

Advantages of using SAS software

Does anybody have experience using SAS software and share its advantages over other softwares like SPSS or Graphpad prism 5 or Stat R? I want to use it for biological research.

06 July 2012 4,344 12 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

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02 March 2021 3,060 3 View

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01 March 2021 3,905 5 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Which group formation theory do you recommend for projects that aim to bring together entrepreneurs for training for regional development?

I would like to have ideas about which theories of group formation are suitable for entrepreneurial training projects, with the aim of creating bonds, building trust, and collaboration for...

28 February 2021 4,223 2 View