I am looking for a lymphocyte cell line that supports the development of merozoites and a tick cell line for the support of sporozoites of theileria.
Tick Cell lines (e.g. Hyalomma Sp.) can be purchased from the tick cell biobank @ University of Edinburgh, UK. (tickcells.roslin.ac.uk)
The tick cell Biobank has moved to the Pirbright Institute:
http://www.pirbright.ac.uk/research/tickcell/Default.aspx
Thanks Pilar for correction and providing new address.
No problem, glad I could help.
I'm working with tick cells HAE CTVM9 and ANE 58 (both From Pirbright Institute). I need to propagate Babesia caballi and Theileria equi in these cells but I have only the merozoites, Does anyone know if this form infect these tick cells?
Dear All, A gene has been cloned in pET alpha 28 to express in BL21. The protein is unable to express. What might be the problem? How can the sequence cloned be verified by any suitable software...
06 July 2014 3,902 8 View
Some laboratories are working on finding benzimidazole drug resistance against haemonchosis in sheep. Workers are finding b-tubulin gene mutation at p200 through gene sequencing. They are actually...
04 May 2014 1,427 1 View
The file is attached for the question cited above 'What statistical software program is needed to draw the graphs and conclusion'
06 July 2013 2,349 44 View
Does anybody have experience of using anti-bovine secondary antibody conjugated with enzyme? I want to use it in ELISA for the sero-diagnosis of parasitic diseases in cattle and buffalo. Does...
06 July 2012 5,888 2 View
Does anybody have experience using SAS software and share its advantages over other softwares like SPSS or Graphpad prism 5 or Stat R? I want to use it for biological research.
06 July 2012 4,453 12 View
I want to explore the long-term effects of incarceration on a youth's developing brain. I also want to explore research that looks critically at incarceration and punitive measures as the primary...
12 August 2024 862 0 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
Please go through my Abstract. I can also share a proposed Thesis Outline.
04 August 2024 2,077 0 View