We are planning to develop method on analysis of Amino Acids in food matrix (Cereals,Pulses , Oil Seeds etc) by HPLC with pre or post column derivatization methods.Since this is new to us so could you please suggest that additional equipment will be required for sample preparations except HPLC i.e. equipments required for hydrolyzing, purifying and concentrating food sample (Cereals, pulses, oilseeds) before injecting into the system for Amino acids analysis.AA
Good evening Dr. Kumar
I think this is quite smart
the attached files may support
best regards
http://www.agilent.com/cs/library/eseminars/public/Column%20Choices%20for%20proteins_037214.pdf
http://www.hplc.eu/Downloads/ACE_Guide_Peptides.pdf
http://www.ijsit.com/admin/ijsit_files/HPLC%20METHOD%20DEVELOPMENT%20FOR%20PROTEINS%20AND%20POLYPEPTIDES_IJSIT_2.4.2.pdf
http://www.waters.com/waters/en_SA/Protein-Separation-Technology-UPLC-and-HPLC-Columns/nav.htm?cid=10073901&locale=en_SA
Dear Santosh,
One problem that we have to solve was the reprudicibility of the derivatization methodology and implemented in loop orthophtaldehyde derivatization (http://dx.doi.org/10.1016/j.chroma.2007.12.014). Best regards
Dear Dr. J.C. Marques,
Thanks for your answer , could you please send me full paper on [email protected] which will help me in analysis of amino acids.
- thanks you but more details are not given in these attachements
Attachments are as follows....Dear Asadollah Sardarzaei ................
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