Can anyone advise on how to do analysis of tylophorine content in crude extract of tylophora indica by HPLC and which column & mobile phase is most suitable?
According to best of my knowledge these are alkaloids. These may be present in S-tylophorine was dextrorotatory and the (–)-isomer had the R configuration. You have separate in Chiral HPLC column because these are soluble in chloroform. You have try with normal phase HPLC system.
Check its solubility again. Then I can give you HPLC method. Try...
Gudluck
:)
Please read following article this may helpful to you
http://phcogj.com/node/621
Extraction process optimization of tylophorine from Tylophora asthmatica Wight & Arn.
Authors and affiliation (s):
1Pharmacognosy division, Department of Pharmaceutical Sciences and Drug Research, Punjabi University, Patiala - 147002.
2NPRD, Panacea Biotec Ltd, Lalru (Punjab) - 140501
Abstract:
Tylophora asthmatica (Synonym- Tylophora indica; family Asclepiadaceae) is a popular traditional remedy for respiratory disorders. The plant contains phenanthroindolizidine type of alkaloids like tylophorine and tylophorinine. The present study was designed to optimize extraction process of tylophorine from T. asthmatica leaves. A series of extraction experiments were carried out using different solvents like 95% alcohol, 2% sulphuric acid solution, chloroform, defatting with petroleum ether then extraction with 95% alcohol, 95% alcohol with different concentrations of glacial acetic acid (1%, 2%, 5% & 10%) and citric acid (0.1%, 0.5%, 1%, 2% & 5%). Maceration method was used for extraction. From all these experiments the extracts obtained were concentrated, purified by acid-base purification technique and tylophorine content was determined. The pure tylophorine fraction was analyzed by HPLC method. Results of the above study show that extraction with 95% alcohol containing 2% citric acid was most effective in terms of higher yield of tylophorine and economy. Hence this method may be used commercially for extraction of tylophorine.
Key words: Tylophora asthmatica, Tylophorine, optimization process.
Thanks all for you valuable suggestion, could you plz share somemore research papers for same analysis
Send ur request on my mail [email protected] i will send u paper related to this
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