I know phi 29 DNA polymerase is sued to amplify the viral genome. Can the Pfu DNA polymerase be used to amplify the viral genome instead of phi 29 DNA polymerase?
PFU can amplify up to 10kb but phi 29 can amplify up to 70kb so it probably depends on the length of your viral genome
Paul Rutland Thank you for the answer. But my doubt is that can the Pfu DNA polymerase amplify the circular ss genome of virus?
I used it for HBV which is circular partially single stranded (Incomplete ds) DNA virus and it works.
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