11 Questions 9 Answers 0 Followers
Questions related from Likhith R K
We have identified consensus sequences from many sequences collected from database. There are "n" in many regions. In order to design primers, what should the n be replaced with?
28 November 2022 6,383 1 View
We want to amplify a viral gene. We are not sure which isolate we will get from our location. So, we have collected some of the reported sequences and carried out multiple sequence alignment. How...
24 November 2022 2,287 2 View
The explants can be regenerated quite easily into a complete plant when they are not infected with Agrobacterium. But, once the explants are infected with Agrobacterium carrying the transgene, the...
16 November 2022 4,402 1 View
I am carrying out single digestion of plasmid followed by alkaline phosphatase treatment to prevent recircularization of the digested ends. So, how long this digested plasmid can be stored?
16 October 2022 4,731 1 View
Has anybody carried out cloning of viral genome using phi 29 DNA polymerase? If so, can you suggest me what are the components required for the process?
02 February 2022 1,138 0 View
I am working on virus and I want to clone the viral genome into a plant for the purpose of screening. Rolling circle amplification (RCA) is one of the methods for cloning viral genome into a...
01 February 2022 7,197 0 View
I know phi 29 DNA polymerase is sued to amplify the viral genome. Can the Pfu DNA polymerase be used to amplify the viral genome instead of phi 29 DNA polymerase?
01 February 2022 7,030 3 View
Biorender is an excellent tool to create illustrations required for seminars and other presentations. But difficult to get the paid version of it. So is there any alternative/similar...
03 January 2022 9,301 2 View
I am trying to transform a gene to a plant. So I am designing a primer for the gene of interest. But the softwares are not giving primers for the full length gene. So, shall I go with manually...
29 December 2021 2,813 10 View
I came across a paper where a gene is being amplified by many pairs of primers. After amplification, all the PCR products were sequenced and were assembled. I wonder what was the reason behind...
09 March 2021 2,468 14 View
I am using BL21 (DE3) cells to study the salt tolerance by transforming my gene into it. So I want to know the tolerance capacity of BL21 (DE3) cells.
19 September 2018 5,624 0 View
