Amplification of constitutive gene and target in same pcr tube can be performed or not ? why not we do RT-PCR like western(same lane with two bands)?

05 May 2015 1 10K Report

Can we do amplification of our target along with constitutive gene in the same tube(both sets of primers in same tube) when their lengths are resolvable ? If yes why it is not practicable(/this avoids technical error and more labour)? if no, why not(/constitutive gene competes with target for PCR components)? I am Curious to know the reason behind the regular practicing of RT-PCR.

Thank you.

Ahmet Buğra Tufan

Yes you can look at the expression of your unknown sample with the expression of a housekeeping gene. System is called as multiplex qrt-pcr by adding two differen primers with two different probes to the master mix.

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