Ahmet Buğra Tufan

3 Questions 5 Answers 0 Followers

Questions related from Ahmet Buğra Tufan

Any suggestions about the Vitamin D Reporter System ?

Hello everybody, We are working with calcitriol (active form of vitamin D3). We gave calcitriol to melanoma cells and looked at gene expression levels of our interested proteins. In the...

06 June 2015 3,815 1 View

Do you have any suggestions on virus toxicity after lentiviral transduction?

I am using lentiviral transduction system. I produce virus in HEK by calcium phosphate method. However, I don't have fluorescent marker. The only marker in my plasmid is neomycin. When I transduce...

06 June 2015 6,477 13 View

Does anyone works with Jurkat cells?

Hello, I want to do western blotting with Jurkat cells. Up to now I did western blotting with solid tumors by using RIPA 0.5-1.0 buffer and 15 minutes of centrifugation at 14.000g. I used the...

06 June 2015 8,238 3 View