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02 March 2021 932 1 View

Tooth extraction and subsequent dental implant placement in Sprague-Dawley rats induce differential changes in anterior digastric myofibre size and ?

01 March 2021 7,254 3 View

Why does intensity drops as concentration increases?

Is there any reason for decrease in fluorescence emission intensity of HSA,BSA when increase the concentration ?

28 February 2021 3,653 4 View

Solution of 2D convection diffusion heat equation using MATLAB ?

This is a polymer heating problem. Polymer enters at around 180C (x=0) in the barrel. Also barrel initial temperature is 180 (t=0). Some heat Is added along whole length of barrel q. We have to...

15 February 2021 3,887 1 View

How is Initial GDP measured ?

Dear All, I am working on estimating Debt-Growth Threshold but confused about some points. My questions are; - How do we calculate initial GDP Per Capita? Shall we do it in STATA or excel file?...

15 February 2021 2,677 2 View

What is the magnetic anisotropy constant for Permalloy (NiFe-80-20)? Can anyone suggest some papers?

Need to know the equation of anisotropy energy for permalloy and also the constants. Any reference paper will be helpful. Thanks for your time and cooperation.

26 January 2021 7,842 3 View

Similarity between these two waveform? Similarity in terms of Shape?

Hi, These are the outputs generated by a system. Green-colored (nr) is desired output, while the system generates Red-colored (nf). I also have a dataset in CSV file. For this waveform, What...

21 January 2021 929 2 View

What are the future research avenues in context of Urban Street, Outdoor thermal comfort and Neural Networks?

Dear academic community, I am a young researcher from architectural background. I am looking for possible novel approaches to evaluate outdoor thermal comfort in urban streets using artificial...

20 January 2021 7,964 3 View

I'm facing some troubling in designing a anteena ....anyone tell me how give CPW feed in cst ?

I want to give a CPW feed anyone suggest how to providea feed in antenna which I'm designed

18 January 2021 3,517 2 View

Can we control the position and vibration, simultaneously, of a flexible link with a tip mass using only the actuator's input?

This is a boundary control problem of a hybrid ODE-PDE system. The system consists of an actuator, a flexible link fixed to the actuator, and a tip mass at the free end of the link. In most...

13 January 2021 8,050 2 View

What is the difference between Q sepharose column and DEAE Sepharose column?

I am working on protein (lipase enzyme) purification. After using the Sephadex G-25 fine column, which column (Q or DEAE sepharose column) should I use? What are the buffers should I select? Thank you

16 February 2021 6,921 5 View

How to purify antibody using immunoaffinity columan ?

we wan to purify Anti-Camel IgG from Poly-clonal serum using Camel IgG couples sepharose.

10 March 2020 1,344 8 View

Can protein binding to Phenyl Sepharose column be done at 1.6 M ammonium sulfate if protein is getting eluted at 1.4 M ?

I am doing HIC for purification of my protein. I am doing binding at 2 M ammonium sulfate and eluting at 1.4 M. Therefore,will lower concentration affect binding or elution of my protein

07 August 2019 5,084 7 View

Protein purification using Con-A column .......?

I am looking for suggestions or comments from protein purification community, especially people who work with Con-A column chromatography. I am used Con-A Sepharose and Con-A agarose beads...

04 May 2019 9,892 6 View

Sepharose beads dried out?

Hi everyone, just yesterday my Prot G Sepharose Beads (Used for immunoprecipitation) dried out. Do you know in which solution they can be re-suspended? I know they are delivered in 20% Ethanol.....

05 April 2019 6,518 4 View

What is the best gel filtration media to remove contaminants from my purified protein ?

We are working on purifying a 60 kDa and have been having issues ridding the fractions of lower MW protein contaminants. We've previously used Ni-NTA and Ni Sepharose 6 fast flow resin for...

02 March 2019 8,184 5 View

Sepharose CL packing in a small column?

Hello, Guys. I'm planning to use Sepharose CL gel for my studies related to liposomes. But, I have no idea how to pack empty PD-10 column (or even smaller) with the Sepharose gel. In the...

02 March 2019 3,769 3 View

How i get clear region between heavy and light chain in control and clear bands in IP lane ?

i am doing immunoprecipitation with flag pull down experiment. And in control pull down with NRS sentacruz. I have obtained smear type region in between heavy and light chain in both IP as well as...

01 February 2019 1,575 2 View

Can a HiPrep 16/60 Sephacryl S-100 column be connected to peristaltic pumps?

Can I connect the column to a peristaltic pump operated at a low flow rate and leave the other end open to elute the contaminants out of the column? Or will it disrupt the column packing??

01 February 2019 9,756 6 View

How to storage the monoclonal antibody after purification in Protein G Sepharose Column?

Monoclonal antibodies produced by hybridomas are purified using Protein G Sepharose Column. The final product is antibodies purified in glycine (pH ~ 2.8) plus Tris-HCl (pH ~ 8.0). My question: If...

01 February 2019 7,084 3 View