Can anyone share a protocol for BS3 covalent crosslinking of Protein A /G Sepharose with primary antibody for CoIP?

29 June 2022 0 9K Report

My target proteins has molecular weight around 50 kDa and 30 kDa. The heavy and light chain of antibody is hiding my target protein bands. I don't have primary and secondary antibody from another host. So covalent crosslinking the antibody to the Protein A/G Sepharose with BS3 seems to be the only option. Can anyone kindly share a protocol for Crosslinking antibody with Protein A/G Sepharose using BS3 crosslinker?

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