Alternative splicing or somatic reversion?

More Luis Ignacio Gonzalez-Granado's questions See All

Is it possible to plot the atom-projected band structure using GPAW?

Hi, I'm currently working on a project where I need to plot the atom-projected band structure using GPAW. I've been able to calculate the band structure for my material, but I'm having trouble...

07 August 2024 269 3 View

In what part of the brain´s rabbit bdnf is located and include references please?

I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....

04 August 2024 762 1 View

How to explain the different interaction between soluble and insoluble polymer in water?

Hello everyone, I am try to understand the differences interaction between insoluble and soluble polymers in water with metal ions and how theit charge density (of metal ions) affect the interaction.

29 July 2024 6,350 3 View

Cuáles fueron las tendencias en investigaciones en arquitectura, urbanismo y patrimonio edificado en decadas del 2000 al 2020?

Cuáles fueron las tendencias en investigaciones en arquitectura, urbanismo y patrimonio edificado en decadas del 2000 al 2020? Porque requiero conocer tesis de posgrado nivel maestría...

24 July 2024 5,494 1 View

Are the coordinates the center of the cell or a corner in GADM / worldclim maps in R?

I am currently using a map downloaded from https://gadm.org/maps/DEU/bayern.html and I would like to assign the value of a variable to each cell with R. I was also trying to get this information...

15 July 2024 8,844 1 View

Can anyone recommend bibliography on the analysis of rage from an affect studies' perspective?

I'm analysing a novel in which the said emotion is fundamental to understand the development of characters. Thank you in advance.

22 June 2024 4,859 8 View

What can a shift in Zn2p edge of ZnO/CuO nanocomposites mean in XPS?

I synthesized pure ZnO with a hydrothermal microwave method and decorated them with CuO with the incipient wetness impregnation method. The Zn2p edges of pure ZnO are at 1044.7 and 1021.6 eV...

18 June 2024 9,405 2 View

Why are my nanoliposomes turning purple??

Hello, I am making lipid nanoparticles using the thin film hydration method. Formula is DODMA:DSPC:Cholesterol:DSG-PEG2000 at 60:20:17.5:2.5 %mM in 5mM total. After hydration in sodium acetate...

16 June 2024 6,842 6 View

Who is the use of Generative Artificial Intelligence in Qualitative Research?

One of the most intriguing frontiers in contemporary research is the application of Generative Artificial Intelligence (GenAI) in qualitative research. This technological advance offers tools that...

13 June 2024 781 2 View

How to normalize fluorescence measurements from different microplate runs?

I'm currently doing an experiment where I'm following the expression of a reporter gene (GFP) along growth using S. cerevisiae. The cultivations last around 48 hours. I observed that the results...

10 June 2024 5,941 3 View

Do I need specific antibodies to stain for a fusion protein?

I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...

07 August 2024 5,338 2 View

Why did the authors extrapolate a phenotype that they experimentally proved in one bacterial strain across the whole genus of the organism?

I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...

05 August 2024 6,787 4 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

Standard curve of H2O2?

Hi, I need to prepare a standard curve of H2O2 to evaluate the primary products of oxidation. H2O2 I have is 35%. How do I prepare a stock of H2O2 10 microlitere. If s.o has experience, I would be...

26 July 2024 3,460 2 View

Can primary care be sustainable ? And how ?

I am starting to work on this question, with particular reference to what we might be able to learn from countries outside the UK. Of course this topic is huge, and I am breaking sustainability...

25 July 2024 1,545 3 View

Trouble finding an anti-Digoxigenin antibody to perform Southern Blot technique?

I'm currently using Roche anti-DIG-AKP Fab fragments to perform Southern Blot assays, but this reagent is delayed since november last year. It's anybody facing the same problem? If so, how are you...

15 July 2024 6,668 0 View

How do I prepare a working stock solution of 14C sodium bicarbonate to get a final activity of 3uCi?

I need to make a stock solution for radioisotopic incubations of freshwater sediments to study dark primary productivity but I have some confusion as to how I calculate the concentration of 14C...

15 July 2024 5,898 0 View

What would you do next?

Studying a specific monogenetic disease based on mutations in different individual genes. I try to recreate the disease phenotypes of donors in vitro. I knocked out a gene x with above 90%...

12 July 2024 6,313 0 View

How to assess the viability and manage debris in fish primary cell cultures?

Hi, I would appreciate professional and technical insights regarding primary cell culturing. I've been working on optimizing my primary cell culture, and while it is successful at times, there are...

10 July 2024 4,918 2 View

How can I avoid uneven IHC staining on free floating tissue from frozen human CNS sections (cryostat 50um thickness)?

Hello, I've been doing free floating IHC using a VAChT antibody with a 1:1k dilution (139 103 - Synaptic Systems). However, there has been a struggle to keep an even stain with my samples of...

06 July 2024 4,521 2 View