How can I convert netcdf file to a binary file in matlab?

Dear all, I have monthly nutrients data from WOA (in a separate NetCDF file) extracted for a specific region. I would like to convert these data directly into a bin file (one of the separate...

30 January 2021 9,241 2 View

My question is that how can we select the unique peptides ?

Hi all Scientists; I have similar question. Can you please help me in selecting unique/distinct peptides in case of LC-MS/MS analysis? I have done salivary gland proteomes for...

26 January 2021 7,488 3 View

How to design reverse primer with ambiguous nucleotides?

How to design, reverse primer with ambiguous nucleotides?

03 January 2021 6,762 4 View

Why does the DNA's elution decrease after centrifuging with spin column?

02 January 2021 987 4 View

How to synthesize pyrrolium ionic salt?

I am keenly interested in the synthesis of heteroaromatic ionic salts for use as an organic template. I want to synthesize alkyl pyrrolium chloride or bromide through a simple addition reaction...

30 December 2020 2,778 3 View

How can large volume ( 100 liters or more ) of carbon dots solution be purified and sieved cost-effectively?

Articles describing the use of carbon dots in agriculture have been increasing recently. Again, studies have found that carbon dots size and shape and some other features are effective. Therefore,...

30 December 2020 1,139 3 View

How to Modeling and simulation for launch vehicle?

Modeling and simulation for launch vehicle Title of graduation project for a master's thesis in mechanical engineering, specializing in Control Search assistance required? Thank you

28 December 2020 9,000 1 View

How to calculate average habitat suitability change between 2 different SDM/ENM?

Hi guys, I would love to know how I could go about solving the above question. I currently have 2 different species distribution models, one created with the current climate projection and the...

14 December 2020 864 8 View

What are good M.Sc thesis topic suggestions in construction management (specially BIM & Waste management area)?

My prefered area is in Waste management & BIM , where I have read about using 4D BIM & 5D in waste management , but I have not reached a significant research gap to work on it, so is...

03 December 2020 1,294 2 View

RBC's contamination during DNA extraction ?

Hi there! currently i am working with spin column kit for DNA extraction I've put almost 600 uL of buffy coat. after a lot of washing, the RBC's remained stucking in filter? How could i get rid...

25 October 2020 8,712 4 View

In PRIMER, is it possible to downweight certain taxa in multivariate community datasets to reflect uncertainty around identification??

I have a dataset with about 80 different species. As usual, some species are very easy to identify with certainty whereas others are more difficult, which means that I am less certain of my...

03 March 2021 8,066 4 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Research on Supply Chain Management in Indian context?

Hi, I am planning to apply for the PhD degree in the Supply Chain Mgt. with specific area of "Cold Storage warehouses" during Pandemics and wars. Where lock downs and shut downs are frequent....

02 March 2021 285 2 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How to calculate the annealing temperature of a primer pair?

I have to amplify a gene and my primers just reached. The Tm for Forward primer is 64.2, and that of reverse primer is 65.5. Can some one suggest how to get the best annealing temperature? Thanks...

01 March 2021 360 7 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

Does any one can help me choose a reasonable basis set for intermediate metal ions interactions with guanine (i.e Fe2+, Mn2+, Cu2+ and exc.)?

Hello every body Does any one has any idea to help me choose a reasonable basis set for intermediate metal ions (i.e Fe2+, Mn2+, Cu2+ and exc.) interactions with guanine or other DNA organic...

01 March 2021 6,187 2 View