Ismail Zeb

7 Questions 8 Answers 0 Followers

Questions related from Ismail Zeb

Is there any manual method for RNA later removal from tissue sample prior to RNA extraction?

06 January 2022 8,604 3 View

What is mean by positive control in PCR validation? Can we use GAPDH primers a house keeping gene for conventional PCR validity?

My negative control in each PCR reaction/ thermocycling was based on not adding cDNA to the reaction. But in the same PCR, I always used the GAPDH housekeeping gene primer. Is it right to use it...

05 January 2022 7,934 3 View

Why PCR is not amplifying the same cDNA on already tested primers which had given results previously?

My cDNA is Pure: 1.89 and quantity: 2525. Previously I used the same cDNA for a pair of primers and got a concentrated band in a 20ul PCR reaction mixture (10uM each working primer), but in the...

10 October 2021 4,360 7 View

How to design reverse primer with ambiguous nucleotides?

How to design, reverse primer with ambiguous nucleotides?

04 January 2021 6,942 4 View

How to open supplementary data in Science Direct?

A supplementary table is present in the paper linked directly to Science Direct, but is not open on that specific site, How to access the attached supplementary file (S1)?

21 September 2020 9,150 2 View

After mRNA to cDNA conversion and then PCR the appearance of PCR bands on non specific position/ near to target region? What is the reason?

To find out the reason why the nonspecific PCR bands appear, either it is due to the annealing of one of the primers?

04 March 2020 2,379 4 View

What will be the sensitivity, specificity, PPV and NPV of two diagnostic tests? Total samples 113 .Microscopy 71 +ive 42 -ive, PCR 110 +ive 3 -ive?

Basically i am working on diagnostic sensitivity of two test, How sensitive and specific PCR is over microscopy.

20 May 2019 2,490 1 View