8 Questions 8 Answers 0 Followers
Questions related from Ismail Zeb
Is there any manual method for RNA later removal from tissue sample prior to RNA extraction?
06 January 2022 8,653 3 View
My negative control in each PCR reaction/ thermocycling was based on not adding cDNA to the reaction. But in the same PCR, I always used the GAPDH housekeeping gene primer. Is it right to use it...
05 January 2022 7,978 3 View
My cDNA is Pure: 1.89 and quantity: 2525. Previously I used the same cDNA for a pair of primers and got a concentrated band in a 20ul PCR reaction mixture (10uM each working primer), but in the...
10 October 2021 4,417 7 View
How to design, reverse primer with ambiguous nucleotides?
04 January 2021 7,071 4 View
A supplementary table is present in the paper linked directly to Science Direct, but is not open on that specific site, How to access the attached supplementary file (S1)?
21 September 2020 9,214 2 View
To find out the reason why the nonspecific PCR bands appear, either it is due to the annealing of one of the primers?
04 March 2020 2,437 4 View
Basically most of the peptides are identified through Liquid chromatography–mass spectrometry So if it is possible to take sequences from this we can align those sequences to find the next area...
22 June 2019 9,414 3 View
Basically i am working on diagnostic sensitivity of two test, How sensitive and specific PCR is over microscopy.
20 May 2019 2,563 1 View
