4 Questions 1 Answers 0 Followers
Questions related from Maxim Dukov
I am using QIAGEN's AllPrep DNA/RNA/miRNA Universal Kit. The handbook doesn’t mention the possibility of additional DNase digestion post-elution, i.e. directly in the RNA solution. Can this...
17 September 2020 7,283 3 View
I'm looking for a formula that calculates minimum coverage needed to call a variant allele with a certain condifence level, given expected variant allele frequency (VAF) (or percentage of mutants...
06 April 2018 9,580 1 View
I’m trying to figure out how many samples per run I can sequence on various platforms for shot-gun metagenome sequencing. I’ve read that 100-150 Gbytes per sample are recommended for WGS of human...
21 March 2018 7,864 4 View
Most reviews or books will tell you that directly-labeled FISH-probes should cover tens of thousands of bases of target DNA in order to be visible in the microscope. But I haven't found any...
18 July 2017 2,828 0 View