Maxim Dukov

4 Questions 1 Answers 0 Followers

Questions related from Maxim Dukov

After extracting RNA with AllPrep RNA/DNA/miRNA Universal kit, can DNase digestion in solution with heat inactivation damage miRNA/lower yield?

I am using QIAGEN's AllPrep DNA/RNA/miRNA Universal Kit. The handbook doesn’t mention the possibility of additional DNase digestion post-elution, i.e. directly in the RNA solution. Can this...

17 September 2020 7,283 3 View

Is there a formula to calculate minimum required coverage for NGS given a certain variant allele frequency and sequencing error?

I'm looking for a formula that calculates minimum coverage needed to call a variant allele with a certain condifence level, given expected variant allele frequency (VAF) (or percentage of mutants...

06 April 2018 9,580 1 View

Any recommendations as to how many bytes/reads/bp per sample are needed for shotgun/16S metagenome sequencing with genus-deep taxonomic profiling ?

I’m trying to figure out how many samples per run I can sequence on various platforms for shot-gun metagenome sequencing. I’ve read that 100-150 Gbytes per sample are recommended for WGS of human...

21 March 2018 7,864 4 View

Is there a review or some other publication that summarizes information on the length of target DNA that indirectly labeled ISH-probes should span?

Most reviews or books will tell you that directly-labeled FISH-probes should cover tens of thousands of bases of target DNA in order to be visible in the microscope. But I haven't found any...

18 July 2017 2,828 0 View