After extracting RNA with AllPrep RNA/DNA/miRNA Universal kit, can DNase digestion in solution with heat inactivation damage miRNA/lower yield?

16 September 2020 7,096 3 View

How to improve Desmond MD/metadynamics calculations performance?

Which program parameters is useful to improve calculations performance (ns/day) of MD simulations without simulation quality loss?

28 June 2020 4,840 2 View

Does it make sense to model mass spectrometric fragmentation by DFT?

Hello. I have met a significant number of publications on the study of the laws of mass fragmentation using DFT. Researchers are building huge schemes where they show in which direction the decay...

05 February 2020 6,252 3 View

How to solve problem with momentum overflow in desmond metadynamics simulations?

During metadynamics calculation, Desmond gives error: -- Allowed momentum exceeded on 2 particles. 7187: force = (-3.0580500e+04 4.4492168e+04 -7.2957642e+03) momentum = (-2.9018593e+01...

03 September 2019 8,099 2 View

Is there a formula to calculate minimum required coverage for NGS given a certain variant allele frequency and sequencing error?

I'm looking for a formula that calculates minimum coverage needed to call a variant allele with a certain condifence level, given expected variant allele frequency (VAF) (or percentage of mutants...

05 April 2018 9,336 1 View

Any recommendations as to how many bytes/reads/bp per sample are needed for shotgun/16S metagenome sequencing with genus-deep taxonomic profiling ?

20 March 2018 7,619 4 View

How much Resazurin powder should be added to 10 ml of culture medium to prepare a solution for a mammalian cells viability assay?

I have Resazurin powder from Sigma but I don't know which concentration must have a stock solution or a final solution for viability test. Please, help me.

05 December 2017 7,371 2 View

Stat analysis on the sequence of events

Hi all, I need an advise on the correct statistical approach for a sequence of events. 1. I have a data collected from a group of individuals at baseline (BL) and follow-up (FU). 2. There are...

04 June 2017 8,081 5 View

Does culturing of ASC lead to a 100% pure collection of ASC? Or does it contain other adherent cell types?

Dear all, I am starting an experiment with adipose derived stem cells (ASC), but I would like some help or confirmation for the following issue. Background: I am harvesting murine fat from the...

10 July 2016 3,373 17 View

No title

18 February 2016 1,339 2 View

Could you recommend any books about Number Theory?

Beginner level books. High level books. Analytical Number Theory. Algebraic Number Theory. Combinatorical Number Theory. Books about partitions. Books about primes.

03 March 2021 7,174 5 View

Hello fellow researcher .. What is the meaning of your name?

I would like to create a small guide containing the meanings of the names .. Thanks to everyone who cooperates with me in achieving it.

03 March 2021 5,266 4 View

What kind of intervention will be relevant to increase motivation among young sport trainees?

Any suggestion on what book to read is also welcome.

03 March 2021 1,646 4 View

How the parameters (such as Mw, Chemical bonds, polymer bland and so on) affect the mechanic property of polymer (such as storage, loss modulus) ?

Anyone can recommend any books on such topics? Thanks in advance.

02 March 2021 8,457 2 View

I am interested in medicinal chemistry field, which books are highly recommended to learn from?

I am at the beginner level in drug design and drug discovery, and I am interested to learn more about this field. Which books are highly recommended to start from and gives me all essential...

02 March 2021 8,437 2 View

Please suggest a good text book for "Research Methodology in Life Sciences". Which book would be good for PhD Scholars?

Please provide Book Title and author name

02 March 2021 9,059 3 View

Which is a lucid standard comprehensive book to guide on psychological scale construction and its standardization?

02 March 2021 7,082 3 View

What element is driving the different slop in the low frequency tail?

My study consists simple corrosion inhibitors in salty brine. Right now I just fit my circuit with randles with CPE. Just wondering how the slop can given more info of the experiment. Or any...

01 March 2021 6,474 3 View

Anyone that can help with details in phosphopeptides enrichment?

Anyone know the amount of protein before enzymatic digestion for phosphopeptides enrichment of serume sample in Ti-IMAC? Has anyone ever use Ti-IMAC(the material is from Chinese company named...

28 February 2021 7,310 3 View

Genome-wide gene identification protocol ?

Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?

28 February 2021 8,095 1 View