Does using increments of 0.5 instead of 1 change the responses to a perceptual scale ?

For example: Would using a 7 point scale of 0 - 3 in steps of 0.5 give you different results to using a 7 point scale of 0 - 6 in steps of 1? I'm aware that verbal labels are likely to be better...

19 November 2020 9,182 5 View

Please suggest any user friendly pipelines for the computation of Polygenic Risk Scores (PRS) on Microarray datasets?

I want to analyze the PRS scores for my microarray datasets, but want to use a platform which is a bit user friendly. Please suggest for any pipelines available.

06 October 2020 5,691 4 View

Is the measurment of DNA concentration and purity necessary for making PCR ? and if it is which is the perfect time to measure?

Nanodrop usually giving unstable records for DNA concentration and purity.

19 July 2020 6,555 5 View

Can any one suggest which solvent is used to coat the calcium phosphate over the Nickel metal dental implant by using spin coating ?

Can anyone suggest which solvent is used to coating the calcium phosphate over the Nickel-metal dental implant by using spin coating?

24 February 2020 8,591 2 View

What is the specific drawing of (?-ES-?) by molecular molecular formula?

Dr. wei, thank you for your reply and your answer is helpful to me. I have put my question in the public Q&A section. In addition, do you know the specific drawing of (?-ES-?), at the...

06 January 2020 7,803 1 View

Can I use Python scripts to get a protein's transient conformation(like open/closed state)?

I want to obtain some protein's open/closed states without using Molecular Dynamics, so is there exists some python scripts to realize my goal? Thank you very much!

10 October 2019 3,303 3 View

How to improve the spreadability of active principle in a membrane forming gel?

Is there any way to prevent the propagation of the thermal effect on the membrane beyond the agitation of the starting gel?

17 October 2018 9,326 1 View

What is the best way to incorporate a drug with better solubility in alkaline solutions into a gel whose thickening is in an acid medium?

I need to deliver a drug of better solubility at alkaline pH, however, the polymer I am working to obtain the gel is thickened at acidic pH. What is the best technology recommended for this case?

21 August 2018 6,162 2 View

Is there corelation between pH, alkalinity and bicarbonate in water?

I have data of pH and total alkalinity of water sample and I want to get bicarbonate value from it.

23 July 2018 9,088 14 View

Why In compound (AgxCu1-x)ZnSnS4 with increasing value of x, lattice constant a increases while c remains unchanged?

11 June 2017 1,187 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View

Compact antenna for the frequency range 1 MHz-5 MHz?

Hello everybody! I have a problem with developing a compact antenna for the 1MHz-5MHz frequency range. Tell me what type of antenna can be used and what books and articles to read to perform this...

27 February 2021 7,710 4 View