Whats the minimum landscape unit for estimating ecosystem service value?

is it possible to estimate ecosystem service value for small units of agricultural land or wetland? . for example, how i can estimate the change is ecosystem services and its values when one...

14 February 2017 4,673 11 View

Can anyone help to identify the attached otolith, fish scale ( may same species) sorted from oceanic squid stomach ?

Can anyone help to identify the attached otolith, fish scale ( may same species) sorted from oceanic squid stomach ?

22 September 2016 5,080 3 View

Among the various globally adopted mix design methods for self compacting concrete which is the most widely used in recent times?

Recent practise of self compacting concrete mix design.

28 October 2014 2,280 2 View

How can I assess the quality of DNA extracted from FFPE tissues?

I have extracted DNA from FFPE tissues (7 - 9 years ago) using Qiagen FFPE kits. I measured the DNA concentration by nanodrop and loaded ~16-20ng DNA to 1.5% gel, but it seems to me there's...

09 October 2014 2,292 4 View

Is there any treatment methods available for plastic coarse aggregates for fire resistance?

Is there any treatment methods available for plastic aggregates (polyethylene) used in concrete for fire resistance? (example: like steel rods are coated to prevent it from corrosion)

01 August 2014 3,427 2 View

Can anyone help with human neutrophil extraction?

I want to isolate human neutrophil after getting the PBMC first. I first put the diluted blood (2ml blood with 2 ml PBS) onto the ficoll paque to extract PBMC. The I took out the bottom red...

28 April 2014 4,210 10 View

Labeling proteins with carboxylate fluospheres using EDC

I am trying to find a working protocol to label some proteins (commercially purchased) with carboxylate modified fluospheres (20 or 40 nm size). I figured out that this can be done using a EDC...

31 May 2013 2,923 2 View

How to match the XRD of a compound for which there is no JCPDS file ? Is there any other characterization to claim the synthesis of desired compound?

I have synthesized LiMgF3 using the starting precursors LiF and MgF2 via solid state diffusion method. Now I am looking for some characterization to claim the desired product, as there is no...

09 February 2021 921 3 View

How to model Type 1 collagen structure for finite element analysis ?

I have started a project in which stress distribution in Type 1-collagen scaffold is to be modelled. I am thinking of using finite element analysis to model the structure and the loading...

07 February 2021 5,487 3 View

Why sometimes current- voltage plot in chemiresistors/ FET does not pass through the origin?

I am trying to measure resistance from I-V curve from a chemiresistor/ FET sensor with a keithly sourcemeter. The substrate is soaked/ wet with protein and phosphate buffer only. But the I-V line...

24 January 2021 8,009 3 View

How can I verify the design on the FPGA ZedBoard?

I have designed AXI Interconnect to connect 4 masters and 1 slave using SystemVerilog and Vivado Tool. The simulation stage of the design is working well. In the design, there are more I/O pins...

06 January 2021 8,487 2 View

Transfecting high molecular weight poly I:C using lipofectamine 2000?

I'm planning to transfect HepG2 and Huh7 with poly I:C (high molecular weight poly IC from Invivogen using lipofectamine 2000 as transfectant reagent. I would like to do a standard curve using...

01 January 2021 239 1 View

Asked a question in SnO2 ?

08 December 2020 6,957 2 View

How to convert Terapeutic Human Doses to Cell Experimentation Doses?

"Toxicological evaluations revealed that Cur is found to be pharmacologically safe, even up to 12 g per day, as reported by several animal studies and in phase-I clinical trials Similarly,...

07 December 2020 3,012 1 View

Any advice on how to get KO cell lines by santa cruz Crispr-cas9 please?

Hello, I tried several times to obtain two KO cell lines for ERN1 (IRE1 alpha) using a santa cruz crispr cas9 "double nickase". (HNSCC cancer cell lines : PECA-PJ41 and PECA-PJ34). I tried...

07 December 2020 8,141 1 View

How to plot a rectification index curve (I/V plot)?

HEK cell is Clamped at different membrane potentials (-100 mV to +90 mV; 10 mv Step with Vh -60 mV) of GluK2 receptor and recorded the current by application of 10 mM L-Glutamate. Herewith I...

07 December 2020 2,002 1 View

How can we make better contacts on thin films for I-V measurement?

Dear researchers, I am currently trying to perform I-V measurements for thin films. Could anybody let me know what is the best way to perform I-V measurements using Keithley 2600. How to make...

01 December 2020 9,103 9 View