Dear Community,
We are looking for an antibody that will bind to the outside of the tertiary structure of AChE. It must not bind to the inside of the tertiary structure of AChE.
So ideally, we need an antibody that meets the following criteria:
• reactivity for both rats and humans
• capable of working in an environment where proteins cannot be denatured
• capable of being used in vivo
• capable of binding to the outside of the 3D structure of AChE
In vivo use in this context does not imply that we intend to inject the antibody into an animal or human, but that we would like to bring it into contact with unfixed rat or human nerve tissue.
Assuming that we would get an antibody custom made: Is it possible to use the same antibody for both rat and human tissue?
Do you see any pitfalls in the experiment that we picture? We are mostly interested in the molecular feasibility.
Any help is highly appreciated!
Hi Richard Schulze ,
Our team would be happy to assist you. We have extensive experience in generating antibodies for in vivo applications with cross-species reactivity.
AChE is a soluble protein and not very hard to produce. Since human and rat AChE share a 88% amino acid similarity, it is highly feasible to develop antibodies able to bind both.
At ProteoGenix, we could develop such an antibody using the hybridoma method with recombinant human AChE (primary antigen) + recombinant rate AChE (secondary antigen). As recombinant AchE are used it means maximized chances of developing antibodies that bind outside of the 3D structure of the native enzyme as can be found in the native nerve tissues.
Our strong expertise in both antibody development and antigen production enables us to proceed quite rapidly since we can produce AchE and perform mouse immunizations in-house. Clone screenings against both human and rat AChE can be done in parallel!
If you work with us, you will retain full intellectual property rights on the antibodies developed for your application. We also provide a guarantee to help you secure your investment.
If you wish to receive a quote for this project, please reach out to my colleagues at the antibody development department and refer this conversation to them: [email protected]
Thank you for considering working with us.
Best regards,
Ana
it is thought to be involved in the pathology of Alzheimer's disease (AD) by accelerating the assembly of Abeta peptides into fibrillar species through forming complexes with Abeta via the peripheral anionic site on ACHE.
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