Is there a way to use waveguide ports for circular antenna design in CST?

Hi, whenever i use circular array i get this error "Waveguide ports must be aligned with Cartesian coordinate planes for the transient solver" Does anyone use circular array with waveguide ports?...

09 February 2021 3,609 8 View

[JAVA]How to fix exception in thread error when using array of objects?

I am doing atm gui for my project in java. In my opinion using array of objects is the best option to store customer data. When i try to access it in an ActionListener method, i get this error:...

06 January 2021 6,975 4 View

How to simulate circular array of antennas in CST?

02 January 2021 7,530 6 View

How can i measure Qmax values of methylene blue adsoprtion correctly?

Hi everyone, I am studying on the adsorption of methylene blue using inorganic based adsorbents. As a result of my study, although the amount of adsorbed dye is approximately 95%, I found that the...

09 September 2020 7,644 4 View

How to understand if CPU is thorttling back?

Hello everyone, When I have tried to solve quick example on Ansys, iterations took too much time and I think my CPU behaved strangely. The frequency is stable but the CPU %use is waving between...

16 March 2019 7,055 3 View

How to prevent carbon cloth/carbon fabric from fraying?

Carbon cloth/carbon fabrics may expose to washing, mixing, hydrothermal and/or high temprature treatments and and consequently they fraying easily. How can i prevent them fraying? Or is there...

19 December 2018 7,083 4 View

Does the ambient temperature (temperature of the lab) affect HPLC operation?

HPLC that I am working with operates in a laboratory which doesn't have any air conditioner and thus, at a temperature of 31 degrees Celsius. Besides, the equipment is exposed to direct sunlight...

30 August 2018 5,086 6 View

Anybody used MethylEdge Bisulfite Conversion System, Promega?

05 December 2016 407 1 View

What are the factors affecting the adhesion strength between metal/oxide and metal/nitride layers?

Microstructure, roughness, the enthalpy of formation of an oxide compared to the adjacent layer are some of the factors affecting the adhesion strength. Is there a specific variable for...

25 September 2014 549 7 View

Is anybody familiar with water removal from nanoporous thin films in the glovebox (N2) by heating? The H2O and O2 content is <1 ppm? The min T needed?

I assume it should be less than in ambient conditions. Since glovebox atmosphere contains trace amounts of O2 and H2O, thermodynamically the temperature needed to evaporate H2O should be less. Do...

21 February 2013 5,731 5 View

Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

How to regulate SSCP temperature in simple PAGE?

I'd like to perform single-strand conformation polymorphism (SSCP) in my thesis, however I cannot control the temperature of the vertical PAGE since we are using the conventional tanks. Is there a...

02 March 2021 9,157 1 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

Do you kow of any database containing data on retention time, log P and polarizability for a large number of molecules?

Hi, I am a chemistry undergraduate doing my dissertation project on HPLC. In my project, I need to predict the HPLC retention time of 20 aromatic molecules, the calibration set. To do this I will...

24 February 2021 2,602 1 View