Is there a way to use waveguide ports for circular antenna design in CST?

Hi, whenever i use circular array i get this error "Waveguide ports must be aligned with Cartesian coordinate planes for the transient solver" Does anyone use circular array with waveguide ports?...

09 February 2021 3,609 8 View

[JAVA]How to fix exception in thread error when using array of objects?

I am doing atm gui for my project in java. In my opinion using array of objects is the best option to store customer data. When i try to access it in an ActionListener method, i get this error:...

06 January 2021 6,975 4 View

How to simulate circular array of antennas in CST?

02 January 2021 7,530 6 View

How to understand if CPU is thorttling back?

Hello everyone, When I have tried to solve quick example on Ansys, iterations took too much time and I think my CPU behaved strangely. The frequency is stable but the CPU %use is waving between...

16 March 2019 7,055 3 View

Does the ambient temperature (temperature of the lab) affect HPLC operation?

HPLC that I am working with operates in a laboratory which doesn't have any air conditioner and thus, at a temperature of 31 degrees Celsius. Besides, the equipment is exposed to direct sunlight...

30 August 2018 5,086 6 View

What are the factors affecting the adhesion strength between metal/oxide and metal/nitride layers?

Microstructure, roughness, the enthalpy of formation of an oxide compared to the adjacent layer are some of the factors affecting the adhesion strength. Is there a specific variable for...

25 September 2014 549 7 View

Is anybody familiar with water removal from nanoporous thin films in the glovebox (N2) by heating? The H2O and O2 content is <1 ppm? The min T needed?

I assume it should be less than in ambient conditions. Since glovebox atmosphere contains trace amounts of O2 and H2O, thermodynamically the temperature needed to evaporate H2O should be less. Do...

21 February 2013 5,731 5 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

In methylene blue test Why is UV absorbance reading very low as soon adsorbent is added and then increasing before reaching equilibrium?

Hello, I'm working on removal of methylene blue. 01. in the dark test very first reading is taken 15 minutes after adding adsorbent to the dye solution and that UV absorbance reading is very...

19 February 2021 472 5 View

Has anyone performed a colorimetric RT-LAMP assay using Lucigens LavaLAMP kit?

Hi there, my lab recently opted to use LavaLAMP from Lucigen instead of the Warmstart kit from NEB. However, Lucigen has yet to create a colormetric kit for RT-LAMP, and adding phenol-Red (pH...

17 February 2021 5,399 1 View

Why stop the reaction in ice for the fluorescent probe FDA (fluorescein diacetate)?

Hi, In literature I noticed that who stains haemocytes with FDA for 30 min then stop the reaction in ice while this does not apply for 15 min incubation time (or maybe authors did not write this...

08 February 2021 3,366 3 View

What is the possible cause of MSCs low growing?

Hello Everyone, We are inexperienced in culture of MSCs trying to grow a sample from BM of a mare. It is the second time that after preparation of new medium (DMEM-F12 with high glucose - 10% Hy...

07 February 2021 9,989 7 View

Why do cells die after adding MTT dye?

I was doing MTT assay, and in the last day before adding the MTT; the cells looked good (or so I think), but after aspiration of the drug and addition of MTT dye I noticed that most cells seemed...

07 February 2021 3,846 4 View

How to measure the concentration of a labeled protein with no tryptophan or tyrosines?

Hey I'm trying to measure the concentration of a protein I've purified and then labeled with Atto 647 flourescent dye. The protein has no tryptophans or tyrosines (although does have...

04 February 2021 4,482 4 View

Why are my western blot bands so distorted?

Hi guys I'm out of ideas here please help! I've never had consistent problem like this doing westerns before and I can't figure it out. The condition used: - First 4 lanes have 50ug protein in...

04 February 2021 2,297 3 View