How should I prepare lignin for atomic force microscopy?

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How can I calculate immobilized enzyme activity?

I immobilized the laccase enzyme with glutaraldehyde on silica airgel support. The protein binds to the support, but its activity is quite low. The color change takes place very little in the...

06 September 2023 1,270 2 View

DisplayPort based on Cyclone 10 GX?

4 transceiver channels are required for DisplayPort. Cyclone 10 GX FPGA; also comes in the form of transceivers BANK. There are 6 transceivers on each bank. A total of 12 Transceivers come in the...

18 April 2021 3,371 0 View

Is there a way to use waveguide ports for circular antenna design in CST?

Hi, whenever i use circular array i get this error "Waveguide ports must be aligned with Cartesian coordinate planes for the transient solver" Does anyone use circular array with waveguide ports?...

09 February 2021 4,315 8 View

SAR analysis problem in CST for antenna arrays?

Hi, i am trying to perform SAR analysis in CST but the problem is after simulation in the post-processing section the SAR tool is not activated. Therefore i cannot get the results. Do you have any...

23 January 2021 8,403 4 View

[JAVA]How to fix exception in thread error when using array of objects?

I am doing atm gui for my project in java. In my opinion using array of objects is the best option to store customer data. When i try to access it in an ActionListener method, i get this error:...

06 January 2021 7,233 4 View

How to simulate circular array of antennas in CST?

Hi, ive desined an array of antennas with 8 elements. When i sımulate it i got this error message in CST "Waveguide ports must be aligned with Cartesian coordinate planes for the transient solve"....

02 January 2021 7,776 6 View

What are the criteria of Kraljic's Purchasing Portfolio Matrix?

Dear All, For my PhD project, I am trying to determine which criteria and factors should be included for two dimensions of Kraljic's Purchasing Portfolio Matrix well-known in the procurement...

11 November 2020 8,301 5 View

How can i measure Qmax values of methylene blue adsoprtion correctly?

Hi everyone, I am studying on the adsorption of methylene blue using inorganic based adsorbents. As a result of my study, although the amount of adsorbed dye is approximately 95%, I found that the...

09 September 2020 7,866 4 View

Acetylation of lignin?

What is the purpose of lignin derivatization with acetic anhydride/pyridine (1/1, v/v) before Gel Permeation Chromatography?

13 October 2019 3,083 4 View

Where can I obtain high purity cheap choline chloride ?

I need to synthesize high volumes of DES with choline chloride.

24 September 2019 3,734 2 View

How can I prepare virus for a TEM or SEM imaging?

I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?

11 August 2024 3,115 1 View

How to increase simulation box size?

We intend to study the interaction between peptides and polymer (like PP, PE and PS) through MD simulations using Martini force fields ( Martini 2 for PP and Martini 3 for PE, PS). We have...

08 August 2024 4,842 0 View

Is there anyone with experience in TEM analysis who can assist with a manuscript for an upcoming journal?

Hello dear colleagues, We have prepared a manuscript on NiTi-based alloys and are seeking a second opinion on our current TEM results. If you are a Ph.D. holder with experience in TEM and have...

07 August 2024 9,563 0 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

Which distribution type should I use when calculating the average particle size from TEM image? and how to calculate the error ?

average particle size calculation from TEM

04 August 2024 2,921 1 View

Is it necessary to covary exogenous constructs in a structural model?

I am working on a SEM model where i have 7 latent variables (6 exogenous and 1 endogenous). In AMOS when I co-vary the exogenous constructs, only 2 paths are coming significant out of 6. But when...

03 August 2024 6,028 4 View

In SEM, if a latent variable consists of three observed variables, and each observed variable has four items, what should I do?

Suppose I am studying the construct of "Academic Motivation" (latent variable) in students, which is measured through three observed variables: "Intrinsic Motivation," "Extrinsic Motivation," and...

31 July 2024 3,913 6 View

Please guide me how to prepare samples for zeiss confocal microscope?

i am working on ischemic stroke. for immunoflourescence i want to use zeiss confocal microscope.before i used VS120 but the images from vs120 not so good now i want to use zeiss confocal but i...

30 July 2024 2,349 1 View

I have synthesized a composite of ZnO and In2O3 with variations in ZnO content. In Raman , what is the peak observed at 130 cm-1?

Dear all, I have noticed, as the ZnO content increases, the Raman intensity at 100cm-1 also increases. However, there is a peak nearby at 130 cm-1, which I could not find in any paper related to...

28 July 2024 5,410 1 View

Bootstrapping in SEM Amos ?

Can a researcher use small sample size of 40 while using SEM with bootstrapping of 1000 and is it possible to get published

28 July 2024 4,402 2 View