I recently came across this article:
Article Pyrophosphate hydrolysis is an intrinsic and critical step o...
According to which the amount of pyrophosphate accumulated during a PCR reaction (or any other in vitro DNA polymerization reaction) should be relatively low, as pyrophosphorolysis, which is the thermodynamic driver of the reaction, seems to be an intrinsic part of the catalytic mechanism of DNA polymerases.
However, the very existence of pyrosequencing, and articles such as:
Article Facilitation of polymerase chain reaction with thermostable ...
where measurement of the amount of pyrophosphate generated during a standard PCR reaction yields values seemingly in the 200-400 uM range, paint a very different picture.
Can anybody explain this apparent contradiction?
Dear Dr. Alejandro Martin
ncbi.nlm.nih.gov/probe/docs/techpcr/
Best Regards
https://sfvideo.blob.core.windows.net/sitefinity/docs/default-source/biotech-basics/the-polymerase-chain-reaction.pdf?sfvrsn=7493407_4
https://sites.google.com/a/hawaii.edu/tpss400/applications/what-is-plateau-effect
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